journal article·2020-07-28

Intrinsic Color Sensing System Allows for Real-Time Observable Functional Changes on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Yiqi Gong, Zhuoyue Chen, Li Yang, Xuefeng Ai, Bingqian Yan, Huijing Wang, Liya Qiu, Yao Tan, Nevin Witman, Wei Wang, Yuanjin Zhao, Wei Fu

ACS Nano· Volume 14· Issue 7
Publisher page

Abstract

Stem-cell based in vitro differentiation for disease modeling offers great value to explore the molecular and functional underpinnings driving many types of cardiomyopathy and congenital heart diseases. Nevertheless, one major caveat in the application of in vitro differentiation of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (hiPSC-CMs) involves the immature phenotype of the CMs. Most of the existing methods need complex apparatus and require laborious procedures in order to monitor the cardiac differentiation/maturation process and often result in cell death. Here we developed an intrinsic color sensing system utilizing a microgroove structural color methacrylated gelatin film, which allows us to monitor the cardiac differentiation process of hiPSC-derived cardiac progenitor cells in real time. Subsequently this system can be employed as an assay system to live monitor induced functional changes on hiPSC-CMs stemming from drug treatment, the effects of which are simply revealed through color diversity. Our research shows that early intervention of cardiac differentiation through simple physical cues can enhance cardiac differentiation and maturation to some extent. Our system also simplifies the previous complex experimental processes for evaluating the physiological effects of successful differentiation and drug treatment and lays a solid foundation for future transformational applications.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Pluripotent Stem Cells Research · 3D Printing in Biomedical Research · Neuroscience and Neural Engineering

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Pediatric Cardiothoracic Surgery, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai 200127, China

Department of Clinical Laboratory, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210008, China

State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China

Department of Anesthesiology, Fudan University Shanghai Cancer Center and Department of Oncology, Shanghai Medical College, Fudan University, 270 Dong An Road, Shanghai 200032, China

Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, School of Medicine, Shanghai Jiao Tong University, 1678 Dong Fang Road, Shanghai 200127, China

Shanghai Institute of Technical Physics of the Chinese Academy of Sciences, Shanghai 200083, China

Department of Medicine and Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm 171 77, Sweden

Shanghai Key Laboratory of Tissue Engineering, Shanghai ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200011, China

Funding

Shanghai Jiao Tong University · Ministry of Science and Technology of the People's Republic of China · Shanghai Jiao Tong University School of Medicine · Shanghai Municipal Human Resources and Social Security Bureau · Science and Technology Commission of Shanghai Municipality · National Natural Science Foundation of China · Shanghai Collaborative Innovation Center for Translational Medicine · Shanghai Key Laboratory of Tissue Engineering, Shanghai Jiao Tong University

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