Real-Time Imaging of Single HIV-1 Disassembly with Multicolor Viral Particles

Yingxin Ma; Zhike He; Tianwei Tan; Wei Li; Zhiping Zhang; Shuang Song; Xiaowei Zhang; Qinxue Hu; Peng Zhou; Yuntao Wu; Xian-En Zhang; Zongqiang Cui

doi:10.1021/acsnano.6b02462

journal article·2016-06-28

Real-Time Imaging of Single HIV-1 Disassembly with Multicolor Viral Particles

Yingxin Ma, Zhike He, Tianwei Tan, Wei Li, Zhiping Zhang, Shuang Song, Xiaowei Zhang, Qinxue Hu, Peng Zhou, Yuntao Wu, Xian-En Zhang, Zongqiang Cui

ACS Nano· Volume 10· Issue 6

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Abstract

Viral disassembly is poorly understood and related to the infection mechanism. However, directly observing the process in living cells remains technically challenging. In this study, the genome RNA, capsid, and matrix protein of the HIV-1 virus were labeled with a Ru(II) complex ([Ru(phen)2(dppz)](2+)), the TC-FlAsH/ReAsH system, and EGFP/ECFP, respectively. Using the multicolored virus and single-particle imaging, we were able to track the sequential disassembly process of single HIV-1 virus particles in live host cells. Approximately 0.1% of viral particles were observed to undergo a sequential disassembly process at 60-120 min post infection. The timing and efficiency of the disassembly were influenced by the cellular factor CypA and reverse transcription. The findings facilitate a better understanding of the processes governing the HIV-1 lifecycle. The multicolor labeling protocol developed in this study may find many applications involving virus-host-cell interactions.

Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: HIV Research and Treatment · Cytomegalovirus and herpesvirus research · Herpesvirus Infections and Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, PR China

Beijing Key Lab of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Sciences, Wuhan University, Wuhan 430072, PR China

National Center for Biodefense and Infectious Diseases, Department of Molecular and Microbiology, George Mason University, Manassas, Virginia 22030, United States

State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, PR China

Funding

Ministry of Science and Technology of the People's Republic of China · National Natural Science Foundation of China · Chinese Academy of Sciences

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