journal article·2019-09-24

Intracellular Photothermal Delivery for Suspension Cells Using Sharp Nanoscale Tips in Microwells

Tianxing Man, Xiongfeng Zhu, Yu Ting Chow, Emma R. Dawson, Ximiao Wen, Alexander N. Patananan, Tingyi Leo Liu, Chuanzhen Zhao, Cong Wu, Jason S. Hong, Pei-Shan Chung, Daniel L. Clemens, Bai-Yu Lee, Paul S. Weiss, Michael A. Teitell, Pei-Yu Chiou

ACS Nano· Volume 13· Issue 9
Publisher page

Abstract

Efficient intracellular delivery of biomolecules into cells that grow in suspension is of great interest for biomedical research, such as for applications in cancer immunotherapy. Although tremendous effort has been expended, it remains challenging for existing transfer platforms to deliver materials efficiently into suspension cells. Here, we demonstrate a high-efficiency photothermal delivery approach for suspension cells using sharp nanoscale metal-coated tips positioned at the edge of microwells, which provide controllable membrane disruption for each cell in an array. Self-aligned microfabrication generates a uniform microwell array with three-dimensional nanoscale metallic sharp tip structures. Suspension cells self-position by gravity within each microwell in direct contact with eight sharp tips, where laser-induced cavitation bubbles generate transient pores in the cell membrane to facilitate intracellular delivery of extracellular cargo. A range of cargo sizes were tested on this platform using Ramos suspension B cells with an efficiency of >84% for Calcein green (0.6 kDa) and >45% for FITC-dextran (2000 kDa), with retained viability of >96% and a throughput of >100 000 cells delivered per minute. The bacterial enzyme β-lactamase (29 kDa) was delivered into Ramos B cells and retained its biological activity, whereas a green fluorescence protein expression plasmid was delivered into Ramos B cells with a transfection efficiency of >58%, and a viability of >89% achieved.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Microfluidic and Bio-sensing Technologies · 3D Printing in Biomedical Research · RNA Interference and Gene Delivery

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States

Department of Pathology and Laboratory Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States

California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California 90095, United States

Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States

Department of Bioengineering, University of California, Los Angeles, Los Angeles, California 90095, United States

Division of Infectious Diseases, Department of Medicine, University of California, Los Angeles, Los Angeles, California 90095, United States

Department of Materials Science and Engineering, University of California, Los Angeles, Los Angeles, California 90095, United States

Molecular Biology Institute, Department of Pathology and Laboratory Medicine, Department of Pediatrics, Jonsson Comprehensive Cancer Center, Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, California 90095, United States

Funding

National Cancer Institute · American Heart Association · Air Force Office of Scientific Research · National Institute on Drug Abuse · Division of Chemical, Bioengineering, Environmental, and Transport Systems · National Institute of General Medical Sciences

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