journal article·2009-02-24

Photothermal Absorption Correlation Spectroscopy

Vivien Octeau, Laurent Cognet, Laurence Duchesne, David Lasne, Nicolas Schaeffer, David G. Fernig, Brahim Lounis

ACS Nano· Volume 3· Issue 2
Publisher page

Abstract

Fluorescence correlation spectroscopy (FCS) is a popular technique, complementary to cell imaging for the investigation of dynamic processes in living cells. Based on fluorescence, this single molecule method suffers from artifacts originating from the poor fluorophore photophysics: photobleaching, blinking, and saturation. To circumvent these limitations we present here a new correlation method called photothermal absorption correlation spectroscopy (PhACS) which relies on the absorption properties of tiny nano-objects. PhACS is based on the photothermal heterodyne detection technique and measures akin FCS, the time correlation function of the detected signals. Application of this technique to the precise determination of the hydrodynamic sizes of different functionalized gold nanoparticles are presented, highlighting the potential of this method.
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Research context

OpenAlex topics: Gold and Silver Nanoparticles Synthesis and Applications · Spectroscopy Techniques in Biomedical and Chemical Research · Advanced Fluorescence Microscopy Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Centre de Physique Moléculaire Optique et Hertzienne, Université de Bordeaux and CNRS, 351 cours de la Libération, Talence, F-33405, France

Centre for Nanoscale Science, School of Biological Sciences, Department of Chemistry, University of Liverpool, Liverpool, L69 7ZB, U.K.

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