journal article·2020-09-22

Multiplexed Graphene Quantum Dots with Excitation-Wavelength-Independent Photoluminescence, as Two-Photon Probes, and in Ultraviolet–Near Infrared Bioimaging

Wen-Shuo Kuo, Xing-Can Shen, Chia-Yuan Chang, Hui-Fang Kao, Sheng-Han Lin, Jiu-Yao Wang, Ping-Ching Wu

ACS Nano· Volume 14· Issue 9
Publisher page

Abstract

In this study, sorted nitrogen-doped graphene quantum dots were prepared and subsequently conjugated with polymers. The synthesized materials exhibited excitation-wavelength-independent photoluminescence emissions ranging from ultraviolet to near-infrared and were 0.9-8.4 nm in size. The materials also exhibited high-photoluminescence quantum yields and excellent two-photon properties. Therefore, in two-photon bioimaging the materials with different emission spectra can be effective two-photon contrast agents. Specific antibodies were used to label organelles in cancer cells and identify nuclear antigens, thereby enabling the simultaneous detection of four targets in cells at a single two-photon excitation wavelength. The sorted nitrogen-doped graphene quantum dot materials were determined to be considerably more advantageous than organic dyes in identifying multiplexed targets, and they can be effective probes in cellular imaging.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Carbon and Quantum Dots Applications · Nanocluster Synthesis and Applications · Graphene and Nanomaterials Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Chemistry and Materials Science, Nanjing University of Information Science and Technology, Nanjing 210044, Jiangsu China

Allergy and Clinical Immunology Research Center, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education), School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China

Department of Mechanical Engineering, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

Department of Nursing, National Tainan Junior College of Nursing, Tainan 700, Taiwan, Republic of China

Department of Anesthesiology, E-Da Hospital, Kaohsiung 824, Taiwan, Republic of China

Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

Department of Biomedical Engineering, National Cheng Kung University, Tainan 701, Taiwan, Republic of China

Funding

E-Da Hospital, Taiwan (R.O.C.) · An Nan Hospital, Taiwan (R.O.C.)

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