DataVXACS NanoPaper
journal article·2024-12-10Open access

QBox: An Automated Portable System for Multiplex Quantum Dot Barcode Diagnostics

Matthew Osborne, Hongmin Chen, Pranav Kadhiresan, Nafisa Mahbub, Ayden Malekjahani, Hannah N. Kozlowski, Buddhisha Udugama, Luan N. M. Nguyen, Stephen Perusini, Samira Mubareka, Warren C. W. Chan

ACS Nano· Volume 18· Issue 49
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Source abstract

Abstract

The COVID-19 pandemic accelerated the development of automated systems for detecting molecular targets for the point-of-care. However, these systems have limited multiplexing capabilities because of the need to alter their hardware to accommodate additional targets and probes. Quantum dot barcodes address this multiplexing obstacle, but their assays have multiple steps that rely on extensive training and laboratory equipment. Here, we built a portable cartridge-and-instrument system that automates the extraction, reverse transcription, amplification, and detection steps of a quantum dot barcode assay. This entire workflow can be completed in 40 minutes. We clinically validated the system with SARS-CoV-2 patient samples ( n = 50, 92% sensitivity, 100% specificity). We then demonstrated multiplexing with 4-barcode respiratory and 5-barcode bloodborne pathogen panels. Our portable system opens quantum dot barcodes for broad use in rapid multiplexed detection of infectious pathogens with the potential for detecting cancer and other genetic diseases.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Biosensors and Analytical Detection · Integrated Circuits and Semiconductor Failure Analysis · Cell Image Analysis Techniques

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario M5S 3E2, Canada

Terrence Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3E1, Canada

MD/PhD Program, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada

Public Health Ontario, Toronto, Ontario M5G 1M1, Canada

Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario M5S 1A8, Canada

Biological Sciences, Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, Canada

Funding

Canadian Institutes of Health Research · University of Toronto · Canada Research Chairs · NanoMedicines Innovation Network · Natural Sciences and Engineering Research Council of Canada · Government of Ontario

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