journal article·2025-06-24

Triple-Cue-Guided Multichannel Hydrogel Conduit to Synergistically Enhance Peripheral Nerve Repair

Yuting Cai, Penghui Wang, Yaxuan Li, Tsz Wing Tang, Linjie Zhang, Hongxin Shu, Hoilun Wong, Yuyin Li, Jingwei Li, Ana Claudia Arias, Chenguang Zhang, Guorui Jin, Qun Huang, Zhengtang Luo

ACS Nano· Volume 19· Issue 24
Publisher page

Abstract

Multichannel nerve guidance conduits (NGCs) have demonstrated superior efficacy in the regeneration of large nerve defects. Here, we present the incorporation of three synergistic guiding cues into a single multichannel hydrogel conduit: topographical guidance, a conductivity gradient, and a nerve growth factor (NGF) gradient. The aligned hydrogel conduit is fabricated through directional lyophilization of a graphene oxide (GO)/poly(vinyl alcohol) (PVA) solution. The conductivity gradient is achieved via the dopamine-induced reduction of GO, during which amino groups with a concentration gradient are simultaneously generated, facilitating the eventual formation of an NGF gradient. In vitro experiments validate the excellent guidance effect and promotion of neuritogenesis by the NGF-gradient/aligned PVA/reduced graphene oxide (rGO)/polydopamine (PDA)/heparin hydrogel conduit (denoted as NGF-AGHC) on PC12 neuronal cells. Furthermore, in vivo testing reveals that NGF-AGHC exhibits a stronger longitudinal attraction to axons and promotes remyelination. Additionally, functional recovery assessments, histological analyses, and morphological evaluations all indicate that NGF-AGHC significantly enhances peripheral nerve regeneration, with performance comparable to that of the autograft group. Altogether, through a straightforward preparation method integrating topographical guidance, conductivity gradients, and NGF gradients, our NGCs offer a promising approach to peripheral nerve repair.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Nerve injury and regeneration · Nerve Injury and Rehabilitation · Hydrogels: synthesis, properties, applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemical and Biological Engineering, and William Mong Institute of Nano Science and Technology, and Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction

The Hong Kong University of Science and Technology

Department of Electrical Engineering and Computer Science

University of California Berkeley

Department of Vascular Surgery, Institute of Vascular Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai 200032, China

Department of Vascular Surgery, Shanghai Ninth People’s Hospital

Shanghai Jiaotong University School of Medicine

Department of Neurosurgery

The Second Affiliated Hospital of Nanchang University

Department of Oral Implantology, Guanghua School of Stomatology, Hospital of Stomatology

Sun Yat-sen University

The Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology

Xi’an Jiaotong University

Funding

Research Grants Council, University Grants Committee · National Key Research and Development Program of China · Innovation and Technology Commission · Fundamental research program funding of Ninth People?s Hospital affiliated to Shanghai Jiao Tong University School of Medicine · International science and technology cooperation projects of Science and Technological Bureau of Guangzhou Huangpu District

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