journal article·2024-04-09

Near-Field Terahertz Morphological Reconstruction Nanoscopy for Subsurface Imaging of Protein Layers

Zhongbo Yang, Dandan Li, Ligang Chen, Fucheng Qiu, Shihan Yan, Mingjie Tang, Chunlei Wang, Lihua Wang, Yang Luo, Fei Sun, Jiaguang Han, Chunhai Fan, Jiang Li, Huabin Wang

ACS Nano· Volume 18· Issue 14
Publisher page

Abstract

Protein layers formed on solid surfaces have important applications in various fields. High-resolution characterization of the morphological structures of protein forms in the process of developing protein layers has significant implications for the control of the layer's quality as well as for the evaluation of the layer's performance. However, it remains challenging to precisely characterize all possible morphological structures of protein in various forms, including individuals, networks, and layers involved in the formation of protein layers with currently available methods. Here, we report a terahertz (THz) morphological reconstruction nanoscopy (THz-MRN), which can reveal the nanoscale three-dimensional structural information on a protein sample from its THz near-field image by exploiting an extended finite dipole model for a thin sample. THz-MRN allows for both surface imaging and subsurface imaging with a vertical resolution of ∼0.5 nm, enabling the characterization of various forms of proteins at the single-molecule level. We demonstrate the imaging and morphological reconstruction of single immunoglobulin G (IgG) molecules, their networks, a monolayer, and a heterogeneous double layer comprising an IgG monolayer and a horseradish peroxidase-conjugated anti-IgG layer. The established THz-MRN presents a useful approach for the label-free and nondestructive study of the formation of protein layers.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Terahertz technology and applications · Plasmonic and Surface Plasmon Research · Photonic and Optical Devices

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Center of Super-Resolution Optics and Chongqing Engineering Research Center of High-Resolution and Three-Dimensional Dynamic Imaging Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China

Chongqing School, University of Chinese Academy of Sciences, Chongqing 400714, China

Institute of Materiobiology, Department of Chemistry, College of Science, Shanghai University, Shanghai 200444, China

Center of Smart Laboratory and Molecular Medicine, School of Medicine, Chongqing University, Chongqing 400044, China

Center for Biological Imaging, Core Facilities for Protein Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Guangxi Key Laboratory of Optoelectronic Information Processing, School of Optoelectronic Engineering, Guilin University of Electronic Technology, Guilin 541004, China

School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200024, China

Funding

University of Chinese Academy of Sciences · National Key Research and Development Program of China · Natural Science Foundation of Chongqing Municipality · National Natural Science Foundation of China

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