Customizable Silk Fibroin-Based Hydrogel Fibrous Scaffold for On-Demand Multifaceted Tissue Repair
Source abstract
Abstract
Hydrogel scaffolds represent an attractive tool for tissue repair. However, targeted tissue repair requires a specific shape and biological function design, and most natural-protein-based hydrogel scaffolds are predominantly confined to specific tissue repair applications. Here, we developed a versatile structural biomimetic natural protein platform through synergistic electrospinning, photopolymerization, and metal-coordination strategies. By integrating methacrylated silk fibroin (SFMA) with acrylated bisphosphonates (AcBP), we developed a dynamically functionalizable matrix that enables (1) customizable shape control via tunable electrospinning collectors and (2) on-demand biological function customization through metal-ion chelation. As a proof of concept, we demonstrate this platform’s scenario-specific therapeutic efficacy: (i) Mg 2+ -functionalized membranes (S-LB-Mg) that orchestrate angiogenic-osteogenic coupling in critical-sized calvarial defects, (ii) Ag + -integrated dressing (S-LB-Ag) enabling bacterial eradication via a nonantibiotic mechanism and accelerating infected wound closure, and (iii) Zn 2+ -loaded conduits (S-LB-Zn) that drive macrophage M2 polarization to enhance peripheral nerve regeneration. This naturally derived protein-based platform overcomes the potential side effects associated with clinical bioactive factor/antibiotic composite scaffolds, offering a simple and customizable solution for the repair and regeneration of diverse tissues in a cost-effective yet highly effective manner. Overall, our strategy provides an alternative perspective for constructing protein-derived hydrogel microfibers with customizable functions and shapes for tissue repair applications.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Silk-based biomaterials and applications · Electrospun Nanofibers in Biomedical Applications · Nerve injury and regeneration
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education, Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology, National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering), School of Biological Science and Medical Engineering
Beihang University
Department of Orthopaedics, Engineering Research Center of Bone and Joint Precision Medicine
Peking University Third Hospital
Key Laboratory of Biorheological Science and Technology Ministry of Education, Collage of Bioengineering
Chongqing University
Department of Orthopedic Surgery, Tongji Hospital
Huazhong University of Science and Technology
Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Nanjing University, 30 Zhongyang Road, Nanjing 210008, Jiangsu, P. R. China
Funding
National Key Research and Development Program of China · Specialized Research Fund for the Doctoral Program of Higher Education of China · Beijing Municipal Science and Technology Commission, Adminitrative Commission of Zhongguancun Science Park · Fundamental Research Funds for the Central Universities · National Natural Science Foundation of China · Higher Education Discipline Innovation Project · International Joint Research Center of Aerospace Biotechnology and Medical Engineering from Ministry of Science and Technology of China
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