journal article·2026-06

Iron‐Based Metal‐Organic Framework MIL‐100(Fe) Regulates Keloid Scarring in a Humanized Keloid Model

Po‐Hsiu Cheng, Dany Y. Matar, Wei‐Ting Chung, Adriana C. Panayi, Yen‐Chang Chen, Yu‐Sheng Yu, Yunching Chen, Tzu‐Lei Kuo, Wen‐Chun Hung, Huang‐Kai Kao, Kevin C.‐W. Wu

Small· Volume 22· Issue 31
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Abstract

ABSTRACT Keloids are characterized by excessive collagen deposition and persistent inflammation, causing high morbidity and recurrence with limited effective treatments. In this study, MIL‐100(Fe)– a metal‐organic framework composed of ferrous/ferric ion nodes and 1,3,5‐benzenetricarboxylate ligands– is synthesized via a microwave‐assisted hydrothermal method and demonstrated nanoscale particle size (141.7 nm). In vitro, human keloid fibroblasts maintained >90% viability after 48 h with MIL‐100(Fe) treatment and showed robust cellular uptake within 2 h, compared to PBS‐treated controls. MIL‐100(Fe) significantly reduced fibroblast migration and downregulated fibrosis‐associated proteins after 48 h, including collagen I, collagen III, transforming growth factor beta 1 (TGF‐β1), SMAD3, and prolyl 4‐hydroxylase subunit alpha 1 (P4HA1). Western blot analysis confirmed that TGF‐β1 expression is more strongly suppressed in human keloid fibroblasts than in human monocytes. In an in vivo humanized keloid mouse model, four weeks of intralesional MIL‐100(Fe) injection reduced fibrous tissue volume by 27% by week two post‐treatment compared with controls. Histological analysis showed decreased fibroblast density, decreased collagen fiber area, polarized macrophage infiltration, and vacuolization in the MIL‐100(Fe) treated group. These findings suggest that MIL‐100(Fe) selectively targets the TGF‐β/SMAD pathway, thereby reducing collagen deposition and fibrosis, and highlight its potential as a therapeutic nanoplatform for keloid treatment.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Dermatologic Treatments and Research · Bone Tissue Engineering Materials · Wound Healing and Treatments

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

International Graduate Program of Molecular Science and Technology Taiwan International Graduate Program Academia Sinica Taipei Taiwan

International Graduate Program of Molecular Science and Technology (NTU‐MST) National Taiwan University Taipei Taiwan

Institute of Biomedical Engineering and Nanomedicine National Health Research Institutes Miaoli Taiwan

Department of Plastic and Reconstructive Surgery Johns Hopkins University School of Medicine Baltimore Maryland USA

Department of Chemical Engineering National Taiwan University Taipei Taiwan

School of Chemical Engineering Purdue University West Lafayette Indiana USA

Department of Cranio‐Maxillofacial and Oral Surgery University Hospital Zurich, University of Zurich Zurich Switzerland

Institute of Biomedical Engineering National Tsing Hua University Hsinchu Taiwan

Department of Chemistry National Tsing Hua University Hsinchu Taiwan

National Institute of Cancer Research National Health Research Institutes Tainan Taiwan

Department of Pharmacy College of Pharmacy, Kaohsiung Medical University Kaohsiung Taiwan

Department of Biological Science and Technology National Yang Ming Chiao Tung University Hsinchu Taiwan

Department of Plastic and Reconstructive Surgery Chang Gung Memorial Hospital & Chang Gung University College of Medicine Taoyuan Taiwan

Department of Chemical Engineering and Materials Science Yuan Ze University Taoyuan Taiwan

Funding

National Science and Technology Council · Chang Gung Memorial Hospital, Linkou · Ministry of Education

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