Fluorescent Polyamines for Efficient DNA Condensation and Cellular Uptake
Source abstract
Abstract
Abstract Polyamine‐based carriers, including spermine and spermidine, offer significant potential due to their ability to bind DNA and facilitate cellular uptake. In this study, four fluorophore‐tagged spermidine and spermine derivatives ( S1C , S2C , S1D , S2D ) linked by a non‐cleavable linker are synthesized and evaluated for their ability to condense DNA and mediate gene delivery. Spectroscopic and bio‐AFM studies confirm the DNA condensation, with S2C exhibiting selective lysosomal targeting and photostability for up to 2 h. Further validation using an RFP‐tagged plasmid confirms S2C ’s effective DNA internalization. This approach offers a promising, straightforward alternative for gene delivery, addressing key limitations of conventional methods. However, further optimization is needed to improve gene release and enhance the therapeutic efficacy.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Polyamine Metabolism and Applications · DNA and Nucleic Acid Chemistry
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Chemistry Indian Institute of Technology Gandhinagar Palaj Gandhinagar Gujarat 382355 India
Funding
Arthritis National Research Foundation
References (40)
Show source-provided references
1. 10.1002/cbic.20180004310.1002/cbic.201800043 ↗
2. 10.1007/s00018-003-2332-410.1007/s00018-003-2332-4 ↗
3. 10.1111/j.1432-0436.1981.tb01123.x10.1111/j.1432-0436.1981.tb01123.x ↗
4. 10.1038/nrd224310.1038/nrd2243 ↗
5. 10.1021/jm301112z10.1021/jm301112z ↗
6. 10.1093/nar/gkf50310.1093/nar/gkf503 ↗
7. 10.1039/D4NJ04965A10.1039/D4NJ04965A ↗
8. 10.1021/acsami.3c0983610.1021/acsami.3c09836 ↗
9. 10.1002/chem.20240107110.1002/chem.202401071 ↗
10. 10.1021/acsabm.9b0008410.1021/acsabm.9b00084 ↗
11. 10.1039/D1NR04328E10.1039/D1NR04328E ↗
12. 10.1021/bc049751710.1021/bc0497517 ↗
13. 10.1021/la00080a03710.1021/la00080a037 ↗
14. 10.1021/bc049957810.1021/bc0499578 ↗
15. 10.1042/bj2002076410.1042/bj20020764 ↗
16. 10.3390/biom1302033710.3390/biom13020337 ↗
17. 10.1016/j.bmcl.2009.03.05210.1016/j.bmcl.2009.03.052 ↗
18. 10.1074/jbc.M40128720010.1074/jbc.M401287200 ↗
19. 10.1021/cb400272m10.1021/cb400272m ↗
20. 10.1016/j.bmcl.2006.03.04610.1016/j.bmcl.2006.03.046 ↗
21. 10.1039/C5CC01637A10.1039/C5CC01637A ↗
22. 10.1016/j.ejmech.2021.11346910.1016/j.ejmech.2021.113469 ↗
23. 10.1021/acs.jmedchem.6b0184610.1021/acs.jmedchem.6b01846 ↗
24. 10.1021/acs.jmedchem.8b0069410.1021/acs.jmedchem.8b00694 ↗
25. 10.1016/j.jconrel.2011.01.01310.1016/j.jconrel.2011.01.013 ↗
26. 10.1016/S0006-3495(99)77002-510.1016/S0006-3495(99)77002-5 ↗
27. 10.1016/0022-2836(78)90367-410.1016/0022-2836(78)90367-4 ↗
28. 10.1073/pnas.050677810210.1073/pnas.0506778102 ↗
29. 10.1007/s00726-016-2246-810.1007/s00726-016-2246-8 ↗
30. 10.1021/acsomega.8b0297710.1021/acsomega.8b02977 ↗
31. 10.1021/jm401174a10.1021/jm401174a ↗
32. 10.1021/bi00316a00210.1021/bi00316a002 ↗
33. 10.1002/(SICI)1097-0282(1997)44:3<269::AID-BIP6>3.0.CO;2-T10.1002/(SICI)1097-0282(1997)44:3<269::AID-BIP6>3.0.CO;2-T ↗
34. 10.1021/bc800035810.1021/bc8000358 ↗
35. 10.1016/S0005-2736(97)00145-410.1016/S0005-2736(97)00145-4 ↗
36. 10.1002/asia.20240060010.1002/asia.202400600 ↗
37. 10.1016/j.matpr.2022.01.22010.1016/j.matpr.2022.01.220 ↗
38. 10.1021/acsnano.9b1021910.1021/acsnano.9b10219 ↗
39. 10.1146/annurev.biochem.78.081307.11054010.1146/annurev.biochem.78.081307.110540 ↗
40. 10.1016/j.cub.2015.02.02710.1016/j.cub.2015.02.027 ↗