DataVXSmallPaper
journal article·2020-07Open access

Enzyme Sampling: Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry (Small 26/2020)

Prithvijit Mukherjee, Eric J. Berns, Cesar A. Patino, Elamar Hakim Moully, Lingqian Chang, S. Shiva P. Nathamgari, John A. Kessler, Milan Mrksich, Horacio D. Espinosa

Small· Volume 16· Issue 26
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Source abstract

Abstract

In article number 2000584, Milan Mrksich, Horacio D. Espinosa, and co-workers report on a method for non-destructively sampling enzymes multiple times from small populations of cells, using localized electroporation in microfluidic live cell analysis devices. The enzymes are captured in extraction chambers, where they modify immobilized substrates on a self-assembled monolayer, and their activity is quantified using self-assembled monolayers for matrix-assisted laser desorption/ionization (SAMDI) mass spectrometry.
Source: openalex_inverted_index · Quality: baseline

Research context

OpenAlex topics: Mass Spectrometry Techniques and Applications · Advanced Proteomics Techniques and Applications · Microbial Inactivation Methods

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Affiliations

Department of Mechanical Engineering Northwestern University Evanston IL 60208 USA

Theoretical and Applied Mechanics Program Northwestern University Evanston IL 60208 USA

Department of Biomedical Engineering Northwestern University Evanston IL 60208 USA

Department of Chemistry Northwestern University Evanston IL 60208 USA

Department of Neurology Northwestern University Feinberg School of Medicine Chicago IL 60611 USA

Department of Cell and Development Biology Northwestern University Feinberg School of Medicine Chicago IL 60611 USA

References

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