Probes: Single‐Cell Detection of mRNA Expression Using Nanofountain‐Probe Electroporated Molecular Beacons (Small 20/2015)
Source abstract
Abstract
H. D. Espinosa and co-workers demonstrate a method for real-time monitoring of gene expression in adherent cells. The method consists of delivering mRNA-detecting molecular beacons into target cells by nanofountain probe electroporation (NFP-E). On page 2386, the NFP-E experimental technique enables temporal studies of the same cell without disturbing cell growth, which could be particularly useful for studies of cell differentiation or for probing specific cells within a cell co-culture population.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Advanced biosensing and bioanalysis techniques
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Affiliations
iNfinitesimal LLC Skokie IL 60077 USA
Department of Mechanical Engineering Northwestern University Evanston IL 60208 USA
Department of Bioengineering University of Pennsylvania Philadelphia PA 19104 USA
Department of Biomedical Engineering Georgia Institute of Technology and Emory University Atlanta GA 30332 USA
References
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