Magnetic Tweezers: Probing DNA Helicase Kinetics with Temperature‐Controlled Magnetic Tweezers (Small 11/2015)
Source abstract
Abstract
The kinetics of double-stranded DNA processing are captured at single-molecule resolution by F. Moreno-Herrero and co-workers with a thermally stabilized magnetic tweezers microscope. An individual protein complex called “AddAB” links one end of a DNA molecule to a superparamagnetic bead that is subjected to an upwards-directed external force. On page 1273, upon addition of the biological fuel ATP, AddAB starts to move along and unwind the two DNA strands, dragging the microsphere behind it. The average translocation velocity increases exponentially with temperature and depends on the ATP concentration.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Bacteriophages and microbial interactions · DNA and Nucleic Acid Chemistry · Genetics, Bioinformatics, and Biomedical Research
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Centro Nacional de Biotecnología, CSIC Darwin 3, Campus de Cantoblanco 28049 Madrid Spain
DNA–Protein Interactions Unit School of Biochemistry Medical Sciences Building University of Bristol University Walk Bristol BS8 1TD UK
References
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