journal article·2014-02Open access

Polymersomes: Third‐Party ATP Sensing in Polymersomes: A Label‐Free Assay of Enzyme Reactions in Vesicular Compartments (Small 3/2014)

Umit Hakan Yildiz, Hans‐Peter M. De Hoog, Zhikang Fu, Nikodem Tomczak, Atul N. Parikh, Madhavan Nallani, Bo Liedberg

Small· Volume 10· Issue 3

Abstract

Polymersomes are encapsulated with a fluorescent reporter and a non-labeled enzyme for sensing of adenosine triphosphate (ATP). As B. Liedberg and co-workers report on page 442, passive diffusion of exogenously added ATP through the membrane is sensed by monitoring the ATPinduced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.
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Research context

OpenAlex topics: Analytical Chemistry and Sensors

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Affiliations

Centre for Biomimetic Sensor Science, School of Materials Science & Engineering Nanyang Technological University 50 Nanyang Drive Singapore 6375532

Institute of Materials Research and Engineering IMRE, 3 Research Link 117602 Singapore

Biomedical Engineering, Chemical Engineering and Materials Science University of California, Davis One Shields Avenue Davis CA 95616 USA

References

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