Bioassays: Eliminating Size-Associated Diffusion Constraints for Rapid On-Surface Bioassays with Nanoparticle Probes (Small 8/2016)

X. Gao and co-workers present a versatile cyclic draining-replenishing (CDR) methodology on page 1035, which effectively “erases” size- associated diffusion constraint characteristics on bulky nanoparticle probes, dramatically enhancing the speed of on-surface binding. Application of CDR to enzyme-linked immunosorbent assays yields reaction-limited assay kinetics with large polymeric enzyme probes. Similarly, a reliable immuno-labeling of cellular targets with fluorescent nanoparticles could be achieved in just 10 min.