Hybrid nanoparticles: Fluorescent–Magnetic Hybrid Nanoparticles Induce a Dose‐Dependent Increase in Proinflammatory Response in Lung Cells in vitro Correlated with Intracellular Localization Small 6/2010

The cover picture shows engineered nanoparticles localized inside a macrophage. Monocultures of human-blood monocyte-derived macrophages are incubated for 24 h with polymer-coated iron platinum nanoparticles, fluorescently labeled with the dye ATTO 590 embedded in the polymer shell (FePt–PMA–ATTO 2% NP). Afterwards the cells were stained with a cell-labeling dye and MitoTracker, which specifically label the mitochondria. The unfixed cells are then imaged by confocal laser scanning microscopy and further processed using a 3D multichannel image-processing software. Nanoparticle agglomerates are shown (red) inside a single macrophage of which the upper half of the cell body is removed (gray). The mitochdondria are shown in blue. For more information, please read the Full Paper “Fluorescent–Magnetic Hybrid Nanoparticles Induce a Dose-Dependent Increase in Proinflammatory Response in Lung Cells in vitro Correlated with Intracellular Localization” by B. Rothen-Rutishauser et al., beginning on page 753.