Bioimaging: Au@pNIPAM SERRS Tags for Multiplex Immunophenotyping Cellular Receptors and Imaging Tumor Cells (Small 33/2015)

Microgel-encapsulated Au nanooctahedra are encoded with various Raman-active dyes by J. Pérez-Juste, L. M. Liz-Marzán, and co-workers. The dyes are resonant with different excitation laser lines, as indicated by the different colors in the image. On page 4149, particles with different SERRS codes are additionally functionalized with specific antibodies toward different cellular receptors, providing multiplex capability for targeted identification of tumor cells in co-culture with non-tumor cells. These highly stable SERRS labels may be applied to high-throughput, multiplex assays for in vitro screening and diagnostics.