A Biochip Model of Lymphocyte Locomotion on Confined Chemokine Tracks

To simulate chemokine tracks presented by narrow stromal networks inside lymph nodes, novel biochips micropatterned with long chemokine stripes are fabricated. Chemokine-coated regions induce T-cell polarization and motility whereas T-cells settling on chemokine-free biochips remain round and static. Chemokine-stimulated T-cell motility is thus directed along patterned stripes (see time-lapse images).