Oxygen Sensing: Ratiometric Nanoparticle Probe Based on FRET‐Amplified Phosphorescence for Oxygen Sensing with Minimal Phototoxicity (Small 32/2020)

In article number 2002494, Andrey S. Klymchenko and co-workers develop a luminescent polymeric nanoparticle probe for oxygen sensing based on the light-harvesting principle that ensures efficient Förster resonance energy transfer from thousands of encapsulated donor fluorescent dyes to few oxygen-sensitive phosphorescent acceptors. Owing to high brightness, color switching in response to oxygen and low phototoxicity, the developed nanoprobe is applied to imaging of microfluidics-generated oxygen gradients in cancer cells. Image credit: Jurga Valanciunaite.