Cellular Patterning Alone Using Bioprinting Regenerates Articular Cartilage Through Native‐Like Cartilagenesis

Abstract Few studies have proved that bioprinting itself helps recapitulate native tissue functions mainly because the bioprinted macro shape can rarely, if ever, influence cell function. This can be more problematic in bioprinting cartilage, generally considered more challenging to engineer. Here a new method is shown to micro‐pattern chondrocytes within bioprinted sub‐millimeter micro tissues, denoted as patterned micro‐articular‐cartilages tissues (PA‐MCTs). Under the sole influence of bioprinted cellular patterns. A pattern scoring system is developed after over 600 bioprinted cellular patterns are analyzed. The top‐scored pattern mimics that of the isogenous group in native articular cartilage. Under the sole influence of this pattern during PA‐MCTs bio‐assembling into macro‐cartilage and repairing cartilage defects, chondrogenic cell phenotype is preserved, and cartilagenesis is initiated and maintained. Neocartilage tissues from individual and assembled PA‐MCTs are comparable to native articular cartilage and superior to cartilage bioprinted with homogeneously distributed cells in morphology, biochemical components, cartilage‐specific protein and gene expression, mechanical properties, integration with host tissues, zonation forming and stem cell chondrogenesis. PA‐MCTs can also be used as osteoarthritic and healthy cartilage models for therapeutic drug screening and cartilage development studies. This cellular patterning technique can pave a new way for bioprinting to recapitulate native tissue functions via tissue genesis.