Probing Single Flavoprotein Molecules on Graphite in Aqueous Solution with Scanning Tunneling Microscopy

Electron tunneling through redox flavoprotein molecules (glucose oxidase) is investigated at the single-molecule level with in situ electrochemical scanning tunneling microscopy (STM; see image). By adjusting the substrate potential, the Fermi levels of the substrate and tip are shifted relative to the energy levels of the proteins, while an increasing tunneling current between the substrate and the STM tip via the flavoprotein molecules is observed. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2296/2008/smll200701041_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.