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journal article·2025-12Open access

Duplex‐Specific DNase Signal Amplification Allows Attomolar Electrochemical Detection of MicroRNAs

Vatsala Pithaih, Henry F. F. Bellette, Thiago C. Pimenta, Dênio E. Pires Souto, Richard W. Tothill, Chamindie Punyadeera, George W. Greene, Conor F. Hogan, Brian Abbey, Saimon M. Silva

Small· Volume 21· Issue 50
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Abstract

Abstract Point‐of‐care biosensors for detecting blood‐circulating nucleic acids hold significant potential for disease diagnosis. However, achieving both high sensitivity and accessibility remains a challenge. Electrochemical hybridization assays offer a promising approach for detecting specific nucleic acid sequences but are typically limited to picomolar concentrations. Here, a duplex‐specific DNase‐assisted electrochemical hybridization assay is presented, enabling highly sensitive nucleic acid detection. By incorporating duplex DNase, the method achieves detection limits as low as 1 attomolar, significantly enhancing sensitivity. This work paves the way for ultrasensitive nucleic acid detection in critical point‐of‐care applications.
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Research context

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · MicroRNA in disease regulation

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

The Biomedical and Environmental Sensor Technology (BEST) Research Centre La Trobe Institute for Molecular Science (LIMS) La Trobe University Melbourne Victoria 3086 Australia

Department of Biochemistry and Chemistry School of Agriculture Biomedicine and Environment La Trobe University Melbourne Victoria 3086 Australia

ARC Research Hub for Molecular Biosensors at Point‐of‐Use (MOBIUS) La Trobe University Melbourne Victoria 3086 Australia

Laboratorio de Espectrometria Sensores e Biossensores ‐ Department of Chemistry Federal University of Paraná (UFPR) Curitiba PR 81530‐900 Brazil

Department of Clinical Pathology and Centre for Genomic Cancer Medicine University of Melbourne Melbourne Victoria 3010 Australia

Sir Peter MacCallum Department of Oncology University of Melbourne Victoria 3010 Australia

Saliva & Liquid Biopsy Translational Laboratory Institute for Biomedicine and Glycomics Griffith University 46, Don Young Rd Brisbane Queensland 4111 Australia

Department of Mathematical and Physical Sciences, School of Computing, Engineering, and Mathematical Sciences La Trobe University Bundoora Victoria 3086 Australia

Funding

National Health and Medical Research Council · Australian Research Council

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