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journal article·2025-05Open access

Functional Microendoscopy Reveals Calcium Responses of Single Cells in Tracheal Tuft Cells and Kidney Podocytes

Tobias A. Dancker, Mohamed Ibrahem Elhawy, Ramona Rittershauß, Qinghai Tian, Yvonne Schwarz, Markus D. A. Hoffmann, Christopher Carlein, Amanda Wyatt, Vanessa Wahl, Daniel Speyerer, Alaa Kandah, Ulrich Boehm, Leticia Prates Roma, Dieter Bruns, Peter Lipp, Gabriela Krasteva‐Christ, Marcel A. Lauterbach

Small· Volume 21· Issue 21
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Source abstract

Abstract

Abstract Microendoscopy, a crucial technology for minimally invasive investigations of organs, facilitates studies within confined cavities. However, conventional microendoscopy is often limited by probe size and the constraint of using a single excitation wavelength. In response to these constraints, a multichannel microendoscope with a slender profile of only 360 µm is engineered. Functional signals both in situ and in vivo are successfully captured from individual single cells, employing a specially developed software suite for image processing, and exhibiting an effective resolution of 4.6 µm, allowing for the resolution of subcellular neuronal structures. This system enabled the first examination of calcium dynamics in vivo in murine tracheal tuft cells (formerly named brush cells) and in situ in kidney podocytes. Additionally, it recorded ratiometric redox reactions in various biological settings, including intact explanted organs and pancreatic islet cultures. The flexibility and streamlined operation of the microendoscopic technique open new avenues for conducting in vivo research, allowing for studies of tissue and organ function at cellular resolution.
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Research context

OpenAlex topics: Ion Transport and Channel Regulation · Pancreatic function and diabetes

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Molecular Imaging Center for Integrative Physiology and Molecular Medicine (CIPMM) Saarland University Kirrberger Str. 100, building 48 66421 Homburg Saarland Germany

Institute of Anatomy and Cell Biology Saarland University Kirrberger Str. 100, building 61 66421 Homburg Saarland Germany

Center for Gender Specific Biology and Medicine (CGBM) Saarland University Kirrberger Str. 100 66421 Homburg Saarland Germany

Molecular Cell Biology, Center for Molecular Signaling (PZMS) Saarland University Kirrberger Str. 100, building 61 66421 Homburg Saarland Germany

Molecular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM) Saarland University Kirrberger Str. 100, building 48 66421 Homburg Saarland Germany

Biophysics, Center for Human and Molecular Biology (ZHMB) Saarland University Kirrberger Str. 100, building 48 66421 Homburg Saarland Germany

Experimental Pharmacology, Center for Molecular Signaling (PZMS) Saarland University Kirrberger Str. 100, building 45&46 66421 Homburg Saarland Germany

Funding

Deutsche Forschungsgemeinschaft · Universität des Saarlandes · Studienstiftung des Deutschen Volkes

References (66)

Show source-provided references
1. 10.1016/j.cgh.2013.06.01510.1016/j.cgh.2013.06.015 ↗
2. 10.1038/s41551-023-01018-010.1038/s41551-023-01018-0 ↗
3. 10.1016/j.yofte.2013.07.00810.1016/j.yofte.2013.07.008 ↗
4. 10.1103/PhysRevLett.109.20390110.1103/PhysRevLett.109.203901 ↗
5. Zhang H. · J. Biomed. Opt. · 2019 · 24
6. 10.1364/BOE.39942810.1364/BOE.399428 ↗
7. 10.1038/srep0180510.1038/srep01805 ↗
8. 10.1364/OE.19.00721310.1364/OE.19.007213 ↗
9. 10.1038/443765a10.1038/443765a ↗
10. 10.1364/BOE.52317910.1364/BOE.523179 ↗
11. 10.1117/1.JBO.23.7.07600510.1117/1.JBO.23.7.076005 ↗
12. 10.1002/smll.20210703210.1002/smll.202107032 ↗
13. 10.1371/journal.pone.004492410.1371/journal.pone.0044924 ↗
14. 10.1117/1.JBO.22.8.08600510.1117/1.JBO.22.8.086005 ↗
15. 10.1364/BOE.7.00340310.1364/BOE.7.003403 ↗
16. 10.1364/OL.33.00133310.1364/OL.33.001333 ↗
17. 10.1364/BOE.44757810.1364/BOE.447578 ↗
18. 10.1038/s41598-020-65950-w10.1038/s41598-020-65950-w ↗
19. 10.1038/s41467-023-36889-z10.1038/s41467-023-36889-z ↗
20. 10.1038/s41566-023-01240-x10.1038/s41566-023-01240-x ↗
21. 10.1159/00048163310.1159/000481633 ↗
22. 10.1038/nrneph.2016.7510.1038/nrneph.2016.75 ↗
23. 10.1016/j.semnephrol.2009.07.01210.1016/j.semnephrol.2009.07.012 ↗
24. Ilatovskaya D. V. · J Vis Exp · 2015
25. 10.1681/ASN.201902010910.1681/ASN.2019020109 ↗
26. 10.1038/s41598-019-44194-310.1038/s41598-019-44194-3 ↗
27. 10.1186/1471-2202-8-4910.1186/1471-2202-8-49 ↗
28. 10.1038/s41598-019-45456-w10.1038/s41598-019-45456-w ↗
29. 10.1164/rccm.201911-2199OC10.1164/rccm.201911-2199OC ↗
30. 10.1073/pnas.101941810810.1073/pnas.1019418108 ↗
31. 10.1096/fj.201901314RR10.1096/fj.201901314RR ↗
32. 10.1172/JCI15095110.1172/JCI150951 ↗
33. 10.1074/jbc.M31284620010.1074/jbc.M312846200 ↗
34. 10.1089/ars.2015.658710.1089/ars.2015.6587 ↗
35. 10.1089/ars.2017.728710.1089/ars.2017.7287 ↗
36. 10.1161/CIRCRESAHA.109.20633410.1161/CIRCRESAHA.109.206334 ↗
37. 10.1126/scisignal.aad389510.1126/scisignal.aad3895 ↗
38. 10.1364/AO.47.00456010.1364/AO.47.004560 ↗
39. Matsushita K. · J Vis Exp · 2018
40. 10.1016/j.mri.2014.07.01210.1016/j.mri.2014.07.012 ↗
41. 10.1021/acsnano.5b0506610.1021/acsnano.5b05066 ↗
42. 10.1016/j.vascn.2019.02.00310.1016/j.vascn.2019.02.003 ↗
43. 10.1161/CIRCULATIONAHA.121.05354710.1161/CIRCULATIONAHA.121.053547 ↗
44. 10.1038/s41598-017-00406-210.1038/s41598-017-00406-2 ↗
45. 10.1681/ASN.201902010910.1681/ASN.2019020109 ↗
46. 10.1017/S143192762000172510.1017/S1431927620001725 ↗
47. 10.1364/OL.33.00133310.1364/OL.33.001333 ↗
48. 10.1117/1.JBO.26.5.05650110.1117/1.JBO.26.5.056501 ↗
49. 10.1364/OL.25.00178010.1364/OL.25.001780 ↗
50. 10.1117/1.JBO.22.3.03600510.1117/1.JBO.22.3.036005 ↗
51. 10.3389/fncel.2019.0014110.3389/fncel.2019.00141 ↗
52. 10.1016/j.isci.2024.10987610.1016/j.isci.2024.109876 ↗
53. 10.1364/OL.52594510.1364/OL.525945 ↗
54. T.Lambert mKate2 at FPbase https://www.fpbase.org/protein/mkate2/(accessed: October 2023).
55. T.Lambert EGFP at FPbase.https://www.fpbase.org/protein/egfp/(accessed: October 2023).
56. 10.1002/gene.1016410.1002/gene.10164 ↗
57. 10.1016/j.neuron.2014.04.03810.1016/j.neuron.2014.04.038 ↗
58. Czogalla J. · J Vis Exp · 2016
59. Plenter R. · J Vis Exp · 2015
60. 10.1093/chemse/bjv02310.1093/chemse/bjv023 ↗
61. 10.1111/j.1440-1681.1982.tb00788.x10.1111/j.1440-1681.1982.tb00788.x ↗
62. 10.1016/0014-2999(79)90160-210.1016/0014-2999(79)90160-2 ↗
63. 10.1152/physiolgenomics.00092.200610.1152/physiolgenomics.00092.2006 ↗
64. 10.3389/fnmol.2022.67424310.3389/fnmol.2022.674243 ↗
65. 10.1371/journal.pbio.300044510.1371/journal.pbio.3000445 ↗
66. 10.1016/0160-5402(78)90022-010.1016/0160-5402(78)90022-0 ↗