journal article·2025-03

Effect of Brief Electrical Stimulation on Cell Biomechanics in Hereditary Sensory Neuropathy

Daniel Ta, Anu Anthony, Ashour Sliow, Boyang Wan, Leo Zhang, Michael Higgins, Lisa Lam, David Mahns, Gaetano Gargiulo, Paul Breen, Damia Mawad, Herleen Ruprai, Simon Myers, Daunia Laurenti, Antonio Lauto

Small· Volume 21· Issue 9
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Abstract

Abstract SH‐SY5Y neuroblastoma cells are widely used to model neurodegenerative disorders like Alzheimer's, Parkinson's, Huntington's, and Hereditary Sensory Neuropathy type 1A (HSN‐1A), a peripheral nerve condition causing axon degeneration and sensory loss. A cell model of HSN‐1A is developed by overexpressing wild‐type and mutant SPTLC1 genes (C133W, C133Y, V144D). Cells are cultured on plastic and gold substrates, with brief electrical stimulation applied to the gold‐grown cells. Atomic force microscopy (AFM) is used to measure Young's modulus, indentation, and energy dissipation. Finite Element Method and non‐linear modeling validate the results. In the absence of stimulation, mutant cells show lower stiffness compared to non‐transfected cells, indicating a direct biomechanical impact of the mutations. Brief electrical stimulation significantly increases the stiffness of mutant cells, particularly in C133W (99%), C133Y (100%), and V144D (111%) variants, despite the mutations. Energy dissipation of stimulated V144D cells decreases to levels comparable to untreated non‐transfected cells. The simulations support the AFM measurements, demonstrating that brief electrical stimulation can partially reverse the biomechanical effects of gene mutations.
Source: crossref · Quality: baseline

Research context

OpenAlex topics: Cellular Mechanics and Interactions · Neuroscience and Neural Engineering · Force Microscopy Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Science Western Sydney University Penrith New South Wales 2751 Australia

School of Aerospace Mechanical and Mechatronic Engineering The University of Sydney Sydney New South Wales 2006 Australia

Centre for Advanced Manufacturing Technology School of Engineering Design and Built Environment Western Sydney University Penrith New South Wales 2751 Australia

ARC Centre of Excellence for Electromaterials Science Intelligent Polymer Research Institute AIIM Facility Innovation Campus University of Wollongong Wollongong New South Wales 2522 Australia

School of Medicine Western Sydney University Penrith New South Wales 2751 Australia

Biomedical Engineering & Neuroscience Research Group The MARCS Institute Western Sydney University Penrith NSW 2751 Australia

The School of Materials Science and Engineering UNSW Sydney Sydney New South Wales 2052 Australia

NICM Health Research Institute Western Sydney University Penrith New South Wales 2751 Australia

Funding

National Health and Medical Research Council

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