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journal article·2025-05Open access

Tetrahedral DNA Nanostructure‐Modified Nanocoating for Improved Bioaffinity and Osseointegration of Titanium

Chenghui Qian, Si Chen, Liman Chen, Chenyang Zhang, Lingyi Yang, Qiaowei Li, Binbin Kang, Xiaohong Chen, Peter Mei, Hongzhou Gu, Yan Liu, Yuehua Liu

Small· Volume 21· Issue 18
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Abstract

Abstract Titanium (Ti) is extensively used in the medical field because of its excellent biomechanical properties; however, how to precisely fabricate Ti surfaces at a nanoscale remains challenging. In this study, a DNA nanocoating system to functionalize Ti surfaces via a series of sequential reactions involving hydroxylation, silanization, and click chemistry is developed. Tetrahedral DNA nanostructures (TDNs) of two different sizes (≈7 and 30 nm) are assembled and characterized for subsequent surface attachment. In vitro and in vivo assays demonstrated significantly enhanced cell adhesion, spreading, proliferation, osteogenesis, and osseointegration on Ti surfaces modified with 30‐nm TDNs, compared to slightly improved effects with 7‐nm TDNs. Mechanistic studies showed that the focal adhesion pathway contributed to the enhanced bioaffinity of the 30‐nm TDNs, as evidenced by the upregulated expression of vinculin and activation of the Akt signaling pathway. Moreover, under inflammatory or hypoxic conditions, Ti surfaces modified with 30‐nm TDNs maintained excellent cellular performance comparable to that under normal conditions, suggesting a broader adaptability for DNA nanoparticles. Thus, better performance is achieved following modification with 30‐nm TDNs. In summary, the proposed DNA‐guided nanocoating system provides a novel and efficient strategy for the surface nanofabrication of Ti.
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Research context

Nanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · RNA Interference and Gene Delivery · Polymer Surface Interaction Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Multidisciplinary Consultant Center Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Shanghai Stomatological Hospital & School of Stomatology Fudan University Shanghai 200001 China

Fudan University Shanghai Cancer Center, the Shanghai Key Laboratory of Medical Epigenetics Institutes of Biomedical Sciences Shanghai Stomatological Hospital Fudan University Shanghai 200433 China

Department of Oral Implantology Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Shanghai Stomatological Hospital & School of Stomatology Fudan University Shanghai 200001 China

Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Fudan University Shanghai 200438 China

School of Materials and Chemistry University of Shanghai for Science and Technology Shanghai 200093 China

Discipline of Orthodontics Department of Oral Science Faculty of Dentistry University of Otago Dunedin 9016 New Zealand

Department of Chemical Biology School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules, and National Center for Translational Medicine Shanghai Jiao Tong University Shanghai 200240 China

Central Laboratory Peking University School and Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of...

Department of Orthodontics Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases Shanghai Stomatological Hospital & School of Stomatology Fudan University Shanghai 200001 China

Funding

National Natural Science Foundation of China · Program of Shanghai Academic Research Leader · Innovative Research Team of High-level Local University in Shanghai

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