Nanopore‐Based Single‐Molecule Logic Unit (sMOLU)

Abstract DNA computing has recently advanced from theoretical computations to a wide variety of applications. Single‐molecule DNA computing combined with nanopore technology offers a unique approach to real‐time molecular computation. A single‐molecule logic unit (sMOLU) is developed that uses α‐hemolysin nanopores to implement a DNA‐based AND logic gate within lipid bilayers. This molecular system leverages a three‐way DNA junction immobilized within a nanopore on a lipid membrane, enabling enzyme‐specific cleavage and signal amplification in giant unilamellar vesicles (GUVs). Fluorescence and electrophysiological measurements confirm precise logic gate operations at the single‐molecule level. The sMOLU platform demonstrates the potential for advancing nanoscale computational systems with precise molecular analysis capability by integrating DNA computing and nanopore technologies.