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journal article·2024-10Open access

Energy Transfer Between i‐Motif DNA Encapsulated Silver Nanoclusters and Fluorescein Amidite Efficiently Visualizes the Redox State of Live Cells

Hari Chandana Yadavalli, Yeolhoe Kim, Il Lae Jung, Sooyeon Park, Tae‐Hwan Kim, Jin Young Shin, Riddhi Nagda, Peter Waaben Thulstrup, Morten Jannik Bjerrum, Yong Joo Bhang, Phil Hyu Lee, Won Ho Yang, Pratik Shah, Seong Wook Yang

Small· Volume 20· Issue 40
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Abstract

Abstract The redox regulation, maintaining a balance between oxidation and reduction in living cells, is vital for cellular homeostasis, intricate signaling networks, and appropriate responses to physiological and environmental cues. Here, a novel redox sensor, based on DNA‐encapsulated silver nanoclusters (DNA/AgNCs) and well‐defined chemical fluorophores, effectively illustrating cellular redox states in live cells is introduced. Among various i‐motif DNAs, the photophysical property of poly‐cytosines (C 20 )‐encapsulated AgNCs that sense reactive oxygen species (ROS) is adopted. However, the sensitivity of C 20 /AgNCs is insufficient for evaluating ROS levels in live cells. To overcome this drawback, the ROS sensing mechanism of C 20 /AgNCs through gel electrophoresis, mass spectrometry, and small‐angle X‐ray scattering is primarily defined. Then, by tethering fluorescein amidite (FAM) and Cyanine 5 (Cy5) dyes to each end of the C 20 /AgNCs sensor, an Energy Transfer (ET) between AgNCs and FAM is achieved, resulting in intensified green fluorescence upon ROS detection. Taken together, the FAM‐C 20 /AgNCs‐Cy5 redox sensor enables dynamic visualization of intracellular redox states, yielding insights into oxidative stress‐related processes in live cells.
Source: crossref · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: Nanocluster Synthesis and Applications · Gold and Silver Nanoparticles Synthesis and Applications · Advanced Nanomaterials in Catalysis

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Systems Biology Institute of Life Science and Biotechnology Yonsei University Seoul 03722 Republic of Korea

Department of Radiation Biology Environmental Radiation Research Group Korea Atomic Energy Research Institute Daejeon 34057 Republic of Korea

Department of Quantum System Engineering Jeonbuk National University Jeonju 54896 Republic of Korea

Department of Neurology College of Medicine Yonsei University 50 Yonsei‐ro, Seodaemun‐gu Seoul 03722 Republic of Korea

Department of Chemistry University of Copenhagen Universitetsparken 5 Copenhagen 2100 Denmark

Xenohelix Research Institute BT Centre 305, 56 Songdogwahak‐ro Yeonsugu Incheon 21984 Republic of Korea

Department of Science and Environment Roskilde University Roskilde 4000 Denmark

Funding

Ministry of Science ICT and Future Planning · Korea Health Industry Development Institute · National Academy of Sciences, Republic of Korea · Chongqing Basic Science and Advanced Technology Research Program · National Agricultural Research Foundation

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