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journal article·2017-05

Microfluidic‐Nanofiber Hybrid Array for Screening of Cellular Microenvironments

Ken‐ichiro Kamei, Yasumasa Mashimo, Momoko Yoshioka, Yumie Tokunaga, Christopher Fockenberg, Shiho Terada, Yoshie Koyama, Minako Nakajima, Teiko Shibata‐Seki, Li Liu, Toshihiro Akaike, Eiry Kobatake, Siew‐Eng How, Motonari Uesugi, Yong Chen

Small· Volume 13· Issue 18
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Source abstract

Abstract

Cellular microenvironments are generally sophisticated, but crucial for regulating the functions of human pluripotent stem cells (hPSCs). Despite tremendous effort in this field, the correlation between the environmental factors—especially the extracellular matrix and soluble cell factors—and the desired cellular functions remains largely unknown because of the lack of appropriate tools to recapitulate in vivo conditions and/or simultaneously evaluate the interplay of different environment factors. Here, a combinatorial platform is developed with integrated microfluidic channels and nanofibers, associated with a method of high‐content single‐cell analysis, to study the effects of environmental factors on stem cell phenotype. Particular attention is paid to the dependence of hPSC short‐term self‐renewal on the density and composition of extracellular matrices and initial cell seeding densities. Thus, this combinatorial approach provides insights into the underlying chemical and physical mechanisms that govern stem cell fate decisions.
Source: crossref · Quality: baseline

Research context

Nanomaterials

OpenAlex topics: 3D Printing in Biomedical Research · Pluripotent Stem Cells Research · Neuroscience and Neural Engineering

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Institute for Integrated Cell‐Material Sciences (WPI‐iCeMS) Kyoto University Yoshida‐Ushinomiya‐cho Sakyo‐ku Kyoto 606‐8501 Japan

Department of Environmental Chemistry and Engineering Graduate School of Interdisciplinary Science and Engineering Tokyo Institute of Technology 4259 Nagatsuta, Midori‐ku Yokohama Kanagawa 226‐8501 Japan

Department of Biomolecular Engineering Graduate School of Bioscience and Biotechnology Tokyo Institute of Technology 259 Nagatsuta, Midori‐ku Yokohama Kanagawa 226‐8501 Japan

Biomaterials Center for Regenerative Medical Engineering Foundation for Advancement of International Science Kasuga, Tsukuba‐shi Ibaraki 305‐0821 Japan

Faculty of Science and Natural Resources Universiti Malaysia Sabah Jalan UMS Kota Kinabalu Sabah 88400 Malaysia

Institute for Chemical Research Kyoto University Gokasho, Uji Kyoto 611‐0011 Japan

Ecole Normale Supérieure CNRS‐ENS‐UPMC UMR 8640 24 Rue Lhomond Paris 75005 France

Funding

Japan Society for the Promotion of Science · New Energy and Industrial Technology Development Organization

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