Protein Immobilization Without Purification via Dip‐Pen Nanolithography
Source abstract
Abstract
Selective immobilization of the histidine (His)-tagged protein from cell lysates is performed on a nitrilotriacetic acid (NTA)/Ni2+ nanopattern fabricated by dip-pen nanolithography (see picture). This pattern and His-coding gene insertions at the end of the target protein gene allow the construction of protein patterns from lysates without prior purification of the protein. LysN = N-terminal domain of lysyl-tRNA synthetase; EGFP = enhanced green fluorescent protein.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Nanofabrication and Lithography Techniques · Monoclonal and Polyclonal Antibodies Research · Force Microscopy Techniques and Applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
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