in situ Transformation of Information Into DNA Storage With Microfluidic Very Large‐Scale Integration Platform

Abstract DNA data storage offers a highly compact and efficient alternative to traditional data storage methods. However, DNA writing remains time‐consuming and relies on specialized infrastructure. Here, a novel, high‐throughput, in situ DNA writing strategy is introduced using microfluidic very‐large‐scale integration (VLSI) chips inspired by dynamic random‐access memory (DRAM) architecture. This method enables rapid encoding of binary data into DNA via overlap‐extension PCR (OE‐PCR) and programmable microfluidic partitioning. Using a widely accessible integrated microfluidic circuit, 2304 bits of data within 4 h encoded, demonstrating scalable, parallelized DNA writing on a benchtop instrument. Next‐generation sequencing confirmed high‐fidelity encoding with excellent signal‐to‐noise ratios. Furthermore, DNA data can be quickly decoded using a microfluidic VLSI qPCR platform, reducing the write‐to‐read latency to under 8 h. This proof‐of‐concept highlights the potential of programmable microfluidic VLSI platforms for efficient, rapid, and portable DNA writing and decoding, paving the way for scalable, high‐throughput, decentralized DNA data storage and gene synthesis.