journal article·2025-11

Nano‐Electro‐Sampling and Intelligent Analysis System for Real‐Time Gene Profiling of Living Cells

Zhaocun Huang, Feng Liu, Deyuan Zhi, Bing Liu, Yihang Tong, Chao Lv, Xi Chen, Shi Yan, Chengbao Wu, Wei Mu, Nan Wu, Lingqian Chang, Zaizai Dong

Small· Volume 21· Issue 46
Publisher page

Abstract

Abstract Accurate intracellular genetic profiling of living cells enables dynamic monitoring of therapeutic response, facilitating timely treatment optimization. However, achieving real‐time gene analysis while maintaining cell viability remains a persistent challenge in existing methodologies. In this work, a Nano‐Electro‐Sampling and Intelligent Analysis (Nesta) system is developed, which incorporates a portable biochip for living cell sampling and on‐chip gene identification with an intelligent image analysis program for accurate gene quantification. The sampling processing utilizes nanopore‐focused electric fields to safely perforate cell membrane and extract intracellular genes. Subsequent sample transfer is effectively realized by chip rotation, which minimizes sample loss and contamination. The detachable detection area satisfies both temperature requirements for gene amplification and cell viability maintenance for sequential sampling. Integrated with intelligent image analysis program, this system enables precise quantitative analysis of smartphone‐captured images. Validated across 50 primary lung cancer cell samples, Nesta system demonstrates over 90% detection concordance with gene sequencing and establishes significant correlation between rebound expression of EGFR mutant genes and acquired drug resistance (AUC = 0.95). This system achieves real‐time gene analysis of living cells, offering a transformative approach in therapeutic monitoring.
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Research context

OpenAlex topics: Microfluidic and Bio-sensing Technologies · Microfluidic and Capillary Electrophoresis Applications · Nanopore and Nanochannel Transport Studies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

School of Engineering Medicine Beihang University Beijing 100191 China

Department of Critical Care Medicine Beijing Friendship Hospital Capital Medical University Beijing 100050 China

Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education Department of Thoracic Surgery II Peking University Cancer Hospital & Institute Beijing 100142 China

Department of Ophthalmology Beijing Friendship Hospital Capital Medical University Beijing 100050 China

School of Biomedical Engineering Research and Engineering Center of Biomedical Materials Anhui Medical University Hefei 230032 China

Qingdao Research Institute Beihang University Qingdao 266100 China

Key Laboratory of Big Data‐Based Precision Medicine (Beihang University) Ministry of Industry and Information Technology of the People's Republic of China Beijing 100191 China

State Key Laboratory of Molecular Oncology Department of Thoracic Surgery II Peking University Cancer Hospital & Institute Beijing 100142 China

Funding

National Major Science and Technology Projects of China · National Science Fund for Distinguished Young Scholars · Key Technologies Research and Development Program · Natural Science Foundation of Shandong Province · National Natural Science Foundation of China · Fundamental Research Funds for the Central Universities

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