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journal article·2022-04Open access

A Self‐Assembled Matrix System for Cell‐Bioengineering Applications in Different Dimensions, Scales, and Geometries

Yong Xu, Michelle Patino Gaillez, Kai Zheng, Dagmar Voigt, Meiying Cui, Thomas Kurth, Lingfei Xiao, Rebecca Rothe, Sandra Hauser, Pao‐Wan Lee, Robert Wieduwild, Weilin Lin, Martin Bornhäuser, Jens Pietzsch, Aldo R. Boccaccini, Yixin Zhang

Small· Volume 18· Issue 13
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Abstract

Abstract Stem cell bioengineering and therapy require different model systems and materials in different stages of development. If a chemically defined biomatrix system can fulfill most tasks, it can minimize the discrepancy among various setups. By screening biomaterials synthesized through a coacervation‐mediated self‐assembling mechanism, a biomatrix system optimal for 2D human mesenchymal stromal cell (hMSC) culture and osteogenesis is identified. Its utility for hMSC bioengineering is further demonstrated in coating porous bioactive glass scaffolds and nanoparticle synthesis for esiRNA delivery to knock down the SOX‐9 gene with high delivery efficiency. The self‐assembled injectable system is further utilized for 3D cell culture, segregated co‐culture of hMSC with human umbilical vein endothelial cells (HUVEC) as an angiogenesis model, and 3D bioprinting. Most interestingly, the coating of bioactive glass with the self‐assembled biomatrix not only supports the proliferation and osteogenesis of hMSC in the 3D scaffold but also induces the amorphous bioactive glass (BG) scaffold surface to form new apatite crystals resembling bone‐shaped plate structures. Thus, the self‐assembled biomatrix system can be utilized in various dimensions, scales, and geometries for many different bioengineering applications.
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Research context

Ceramics & glassesNanomaterialsBiomaterials

OpenAlex topics: Bone Tissue Engineering Materials · 3D Printing in Biomedical Research · Additive Manufacturing and 3D Printing Technologies

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

B CUBE Center for Molecular Bioengineering Technische Universität Dresden 01307 Dresden Germany

Institute of Biomaterials Department of Materials Science and Engineering University of Erlangen‐Nuremberg 91058 Erlangen Germany

Institute of Botany Faculty of Biology Technische Universität Dresden 01062 Dresden Germany

Technische Universität Dresden Center for Molecular and Cellular Bioengineering (CMCB) Technology Platform EM Facilty 01307 Dresden Germany

Department of Spine Surgery and Musculoskeletal Tumor Zhongnan Hospital of Wuhan University Wuhan University Wuhan 430071 China

Helmholtz‐Zentrum Dresden‐Rossendorf (HZDR) Institute of Radiopharmaceutical Cancer Research Department of Radiopharmaceutical and Chemical Biology 01328 Dresden Germany

Technische Universität Dresden School of Science Faculty of Chemistry and Food Chemistry 01062 Dresden Germany

Center for Regenerative Therapies Dresden (CRTD) Technische Universität Dresden Fetscherstraße 105 01307 Dresden Germany

University Hospital Carl Gustav Carus der Technischen Universität Dresden Medizinische Klinik und Poliklinik I Fetscherstraße 74 01307 Dresden Germany

Cluster of Excellence Physics of Life Technische Universität Dresden 01062 Dresden Germany

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