journal article·2021-01

Optical Imaging and High‐Accuracy Quantification of Intracellular Iron Contents

Dewen Ye, Mingxi Li, Yuanyuan Xie, Bo Chen, Yuexia Han, Sijin Liu, Qi‐Huo Wei, Ning Gu

Small· Volume 17· Issue 2
Publisher page

Abstract

Abstract Precise quantification of intracellular iron contents is important to biomedical applications of magnetic nanoparticles. Current approaches for iron quantification rely on specialized instruments while most only yield iron quantities averaged over plenty of cells. Here, a simple and robust approach, combining digital optical microscopy with the Beer–Lambert's law, that allows for imaging stainable iron distribution in individual cells and the quantification of stainable iron contents with an unprecedented accuracy of femtogram per pixel, is presented. It is further shown that this approach enables studying of the internalization and reduction dynamics of super‐paramagnetic iron oxide nanoparticles (SPIONs) by stem cells in single cell level.
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Research context

OpenAlex topics: Microfluidic and Bio-sensing Technologies · Characterization and Applications of Magnetic Nanoparticles · Electrostatics and Colloid Interactions

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and Devices School of Biological Science & Medical Engineering Southeast University Nanjing 210009 China

Center for Clinic Stem Cell Research The Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 China

Materials Science and Devices Institute Suzhou University of Science and Technology 1 Kerui Road Suzhou 215009 China

State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco‐Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

Department of Mechanical and Energy Engineering Southern University of Science and Technology Shenzhen 518055 China

Department of Physics Nanjing Medical University Nanjing 211166 China

Funding

National Basic Research Program of China · National Natural Science Foundation of China · Foundation for Innovative Research Groups of the National Natural Science Foundation of China · National Key Research and Development Program of China Stem Cell and Translational Research

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