De Novo Engineering of Metal–Organic Framework‐Printed In Vitro Diagnostic Devices for Specific Capture and Release of Tumor Cells

Abstract Herein, a paper‐based in vitro diagnostic device (IVD) is developed via inkjet printing of de novo engineered, boronic acid‐rich metal–organic frameworks (BMOFs). The newly developed BMOFs simultaneously possess crystalline and amorphous structure, mesopore size, large surface area, and retain a high level of boronic acid integration. After printing the BMOFs on the filter paper, the BMOF‐printed paper IVD shows a rapid response time (40 min) towards cancer cell capture and its maximum cell capture capacity reaches approximately (4.5 ±1.1) ×10⁴ cells cm⁻². Furthermore, the BMOF‐printed IVD shows nine times higher capture ability of cancer cells than non‐cancerous cells, suggesting its excellent selectivity. Importantly, the pH‐tunable affinity of BMOF to glucose enables its dual‐responsive behavior without affecting cell viability. In addition, a desired cell pattern could be achieved by directly drawing BMOFs onto a silicon substrate, highlighting its capacity as a miniaturized device for tumor cell capture and analysis. This simple and label‐free nanoplatform enables new opportunities for designing MOF‐based smart devices for diverse biomedical applications such as a cost‐effective IVD technologies for cancer diagnosis, genotyping, and prognosis.