Pyrrole/Imidazole–Polyamide Anchors for DNA Tertiary Interactions

Tertiary interactions are introduced for DNA nanotechnology by covalently coupling a sequence-specific DNA-binding polyamide (“anchor”) to an oligonucleotide. By ligating this DNA–polyamide hybrid into a double-stranded DNA circle that carries the binding site for the polyamide, the circles self-assemble to form stable homodimeric complexes that are imaged by atomic force microscopy (see image). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.