Compact Halo‐Ligand‐Conjugated Quantum Dots for Multicolored Single‐Molecule Imaging of Overcrowding GPCR Proteins on Cell Membranes
Source abstract
Abstract
To detect single molecules within the optical diffraction limit (< ca. 200 nm), a multicolored imaging technique is developed using Halo-ligand conjugated quantum dots (Halo-QDs; <6 nm in diameter). Using three types of Halo-QDs, multicolored single-molecule fluorescence imaging of GPCR proteins in Dictyostelium cells is achieved.
Source: openalex_inverted_index · Quality: baseline
Research context
OpenAlex topics: Quantum Dots Synthesis And Properties · Advanced biosensing and bioanalysis techniques · Molecular Junctions and Nanostructures
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Laboratory for Nano‐Bio Probes Quantitative Biology Center, Riken Suita 565–0874 Osaka Japan
Laboratory for Cell Signaling Dynamics Quantitative Biology Center, Riken Suita 565–0874 Osaka Japan
Laboratory of Single Molecule Biology Department of Biological Sciences Graduate School of Science Osaka University Toyonaka 560–0043 Osaka Japan
References (37)
Show source-provided references
1. 10.1146/annurev.biophys.26.1.37310.1146/annurev.biophys.26.1.373 ↗
2. 10.1146/annurev.biochem.77.070606.10154310.1146/annurev.biochem.77.070606.101543 ↗
3. 10.1126/science.281.5385.201310.1126/science.281.5385.2013 ↗
4. 10.1126/science.281.5385.201610.1126/science.281.5385.2016 ↗
5. 10.1126/science.110427410.1126/science.1104274 ↗
6. 10.1038/nbt76410.1038/nbt764 ↗
7. 10.1038/nbt76710.1038/nbt767 ↗
8. 10.3390/s9110933210.3390/s91109332 ↗
9. 10.1126/science.108852510.1126/science.1088525 ↗
10. 10.1038/nbt92910.1038/nbt929 ↗
11. 10.1073/pnas.070253610410.1073/pnas.0702536104 ↗
12. 10.1523/JNEUROSCI.1825-06.200610.1523/JNEUROSCI.1825-06.2006 ↗
13. 10.1242/jcs.00735110.1242/jcs.007351 ↗
14. 10.1529/biophysj.106.08483010.1529/biophysj.106.084830 ↗
15. 10.1529/biophysj.106.09489610.1529/biophysj.106.094896 ↗
16. 10.1182/blood-2009-02-20545010.1182/blood-2009-02-205450 ↗
17. 10.1038/nmeth.144410.1038/nmeth.1444 ↗
18. Technical Manual Promega “HaloTag Technology: Focus on Imaging” http://www.promega.com 2013 accessed: August 2014.
19. 10.1021/cb800025k10.1021/cb800025k ↗
20. 10.1021/nn304793z10.1021/nn304793z ↗
21. 10.1002/anie.20060119710.1002/anie.200601197 ↗
22. 10.1016/j.bbrc.2008.07.00410.1016/j.bbrc.2008.07.004 ↗
23. 10.1126/science.106395110.1126/science.1063951 ↗
24. Miyanaga Y. · Methods Mol. Biol. · 2009 · 571 · 41710.1007/978-1-60761-198-1_28 ↗
25. 10.1126/science.304787110.1126/science.3047871 ↗
26. 10.1021/jp971091y10.1021/jp971091y ↗
27. 10.3390/ijms910204410.3390/ijms9102044 ↗
28. 10.1039/b812302k10.1039/b812302k ↗
29. 10.1039/b921602b10.1039/b921602b ↗
30. 10.1039/c3cc44000a10.1039/c3cc44000a ↗
31. 10.1039/C2CC36870F10.1039/C2CC36870F ↗
32. 10.1039/c2ay25318f10.1039/c2ay25318f ↗
33. 10.1038/383802a010.1038/383802a0 ↗
34. 10.1126/scisignal.200098010.1126/scisignal.2000980 ↗
35. 10.1016/j.bpj.2012.09.01410.1016/j.bpj.2012.09.014 ↗
36. 10.1016/j.bpj.2010.07.03610.1016/j.bpj.2010.07.036 ↗
37. 10.1111/dgd.1206410.1111/dgd.12064 ↗