Fiber‐Optic High‐Resolution Genetic Screening Using Gold‐Labeled Gene Probes

A new, ultra-sensitive genetic screening tool based on a fiber-optic (FO) surface plasmon biosensor and gold-labeled gene probes is presented. The assay allows for the detection and location of single-point mutations (SNP) at significantly reduced cost and high speed. The assay is evaluated for SNP detection of 3 different DNA targets, including a GC-rich cancer gene fragment and a long bacterial sequence. The data proves that the accuracy of the FO-SNP test is as good or, in some applications, better than classical high-resolution melting (HRM). Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.