journal article·2008-03-03

Stepwise Self‐Assembly of a Protein Nanoarray from a Nanoimprinted Poly(Ethylene Glycol) Hydrogel

Bong Kuk Lee, Hea Yeon Lee, Pilnam Kim, Kahp Y. Suh, Jeong Hyun Seo, Hyung Joon Cha, Tomoji Kawai

Small· Volume 4· Issue 3
Publisher page

Abstract

Ultraviolet nanoimprint lithography is used to fabricate nanopatterns of poly(ethylene glycol) (PEG) hydrogel on gold substrates for the construction of protein nanoarrays. A protein nanoarray with a minimum feature size of 100 nm is constructed in the nanowell of the PEG hydrogel by self-assembly with a biotinylated PEG-disulfide, streptavidin, and biotinylated antibody, sequentially (see figure), and confirmed by varying the nanowell height between each step in both solution and air.
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Research context

OpenAlex topics: Nanofabrication and Lithography Techniques · Advanced Biosensing Techniques and Applications · Monoclonal and Polyclonal Antibodies Research

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References (42)

Show source-provided references
1. 10.1016/S0006-3495(95)80394-210.1016/S0006-3495(95)80394-2 ↗
2. Ekins R. · J. Int. Fed. Clin. Chem. · 1997 · 9 · 100
3. 10.1038/1509010.1038/15090 ↗
4. 10.1038/7444210.1038/74442 ↗
5. 10.1126/science.289.5485.176010.1126/science.289.5485.1760 ↗
6. 10.1016/S1367-5931(00)00170-810.1016/S1367-5931(00)00170-8 ↗
7. 10.1093/clinchem/47.10.189410.1093/clinchem/47.10.1894 ↗
8. 10.1002/1615-9861(200110)1:10<1271::AID-PROT1271>3.0.CO;2-610.1002/1615-9861(200110)1:10<1271::AID-PROT1271>3.0.CO;2-6 ↗
9. 10.1038/ng103710.1038/ng1037 ↗
10. 10.1038/nbt0402-35910.1038/nbt0402-359 ↗
11. 10.1126/science.290.5496.153210.1126/science.290.5496.1532 ↗
12. 10.1146/annurev.bioeng.4.112601.12591610.1146/annurev.bioeng.4.112601.125916 ↗
13. 10.1039/b103795c10.1039/b103795c ↗
14. 10.1021/ac034640710.1021/ac0346407 ↗
15. Ekins R. P. · Clin. Chem. · 1998 · 44 · 201510.1093/clinchem/44.9.2015 ↗
16. 10.1146/annurev.bioeng.2.1.55110.1146/annurev.bioeng.2.1.551 ↗
17. 10.1126/science.290.5496.153610.1126/science.290.5496.1536 ↗
18. 10.1021/ja00076a03810.1021/ja00076a038 ↗
19. 10.1021/la980642o10.1021/la980642o ↗
20. 10.1021/la000954310.1021/la0009543 ↗
21. 10.1021/la991196n10.1021/la991196n ↗
22. 10.1021/la010074410.1021/la0100744 ↗
23. 10.1126/science.106717210.1126/science.1067172 ↗
24. 10.1016/0003-2670(92)80002-O10.1016/0003-2670(92)80002-O ↗
25. 10.1021/la971331110.1021/la9713311 ↗
26. 10.1063/1.11062810.1063/1.110628 ↗
27. 10.1016/S0142-9612(99)00165-910.1016/S0142-9612(99)00165-9 ↗
28. 10.1021/ac035415s10.1021/ac035415s ↗
29. 10.1039/B512593F10.1039/B512593F ↗
30. 10.1021/nl049758x10.1021/nl049758x ↗
31. 10.1021/nl048943810.1021/nl0489438 ↗
32. 10.1016/S0167-9317(03)00078-910.1016/S0167-9317(03)00078-9 ↗
33. 10.1021/la026967t10.1021/la026967t ↗
34. 10.1002/jbm.b.3034810.1002/jbm.b.30348 ↗
35. 10.1039/b610503c10.1039/b610503c ↗
36. 10.1021/la700768310.1021/la7007683 ↗
37. 10.1016/S0167-9317(00)00304-X10.1016/S0167-9317(00)00304-X ↗
38. 10.1021/la010075w10.1021/la010075w ↗
39. 10.1021/la035129b10.1021/la035129b ↗
40. 10.1021/ac061762110.1021/ac0617621 ↗
41. 10.1016/0076-6879(90)84259-J10.1016/0076-6879(90)84259-J ↗
42. 10.1021/la990807910.1021/la9908079 ↗