Probing the Biological Identity of Inorganic Nanoparticles with Anomalous Small Angle X‐Ray Scattering
Source abstract
Abstract
Abstract In a biological milieu, nanoparticles (NPs) undergo alterations that go from aggregation or degradation to the adsorption of a corona of proteins. Those changes influence significantly the intrinsic properties and biological capacities of the engineered nanomaterials and need to be fully understood for the successful biomedical use of NPs. Synchrotron anomalous small‐angle X‐ray scattering (ASAXS) is used to probe the individual small‐angle X‐ray scattering (SAXS) contributions from the different components of NPs (core, polymeric coating, and protein corona) on several in vitro systems. By applying a model‐based fitting approach combined with pair distance distribution analysis, it is possible to investigate whether and to what extent proteins formed a corona around different nanoparticles. The results obtained agree with well‐established dynamic light scattering and transmission electron microscopy experiments, confirming the capacity of ASAXS to obtain full information from complex NP samples, and opening for its use in more realistic biological environments.
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Research context
OpenAlex topics: Nanoparticle-Based Drug Delivery · Iron oxide chemistry and applications · Nanoparticles: synthesis and applications
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Center for Hybrid Nanostructures University of Hamburg 22761 Hamburg Germany
The Hamburg Centre for Ultrafast Imaging 22761 Hamburg Germany
Center for Cooperative Research in Biomaterials (CIC biomaGUNE) Basque Research and Technology Alliance (BRTA) Donostia‐San Sebastian 20014 Spain
Deutsches Elektronen‐Synchrotron DESY Notkestr. 85 22607 Hamburg Germany
Ikerbasque, Basque Foundation for Science Bilbao 48013 Spain
Donostia International Physics Center Donostia‐San Sebastian 20018 Spain
Polimero eta Material Aurreratuak: Fisika, Kimika eta Teknologia Kimika Fakultatea Euskal Herriko Unibertsitatea UPV/EHU Donostia‐San Sebastian 20018 Spain
Funding
Deutsche Forschungsgemeinschaft · Agencia Estatal de Investigación · 'la Caixa' Foundation · Diputación Foral de Gipuzkoa · HORIZON EUROPE European Research Council · H2020 European Research Council
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