A Biomimetic Hierarchical Porous Microneedle for Transdermal Ovarian Cancer Detection

Abstract Precision in early‐stage ovarian cancer detection is crucial for improving survival rates, yet often involves invasive or resource‐intensive procedures. It is here engineered a portable transdermal biosensor comprising antibody‐functionalized hierarchical porous microneedles (hp‐MNs) that enables on ‐needle quantitatively detect human epididymis protein 4 (HE4) and cancer antigen 125 (CA125) via fluorescence immunoassay. Fabricated through a novel phase separation micro‐molding technique, the biomimetic microneedles feature a coral‐like hierarchical inter‐connective porous structure with noteworthy liquid absorption capacity and high antibody‐binding density, enabling reliable in vivo biomarkers enrichment and detection with nanomolar sensitivity to differentiate disease from benign biofluids. Furthermore, a groove structure allows concurrently detection of multiple tumor biomarkers at identical regions without cross‐influence in a single application, enhancing sensitivity and precision. This work advances the field of porous polymeric microneedles and offers a promising guideline for developing more accurate and noninvasive diagnostic systems.