Enhancing Diabetic Oral Wound Healing with miR‐132 Delivered Through Tetrahedral DNA Nanostructures

Abstract Oral mucosal injuries are commonly caused by factors such as trauma, infection, or inflammation, especially in diabetic patients where healing is difficult and significantly affects quality of life. In this study, a nanocarrier system based on DNA tetrahedrons (TDN) is developed, which serve as ideal vectors due to their excellent intracellular uptake and drug delivery capabilities. By efficiently delivering miR132 into cells, the proliferation and migration of human oral mucosal fibroblasts (HOMFs) and human umbilical vein endothelial cells (HUVECs) are regulated, along with the modulation of inflammation and antioxidant processes. In the oral wound model of diabetic rats, the miR@TDN system effectively and stably delivers miR132 to the injured mucosa. By regulating the inflammatory response, promoting blood vessel regeneration, and enhancing antioxidant defense mechanisms, significant improvement in cellular repair function and acceleration of the wound healing process are observed. These findings provide a new strategy and experimental basis for the clinical treatment of oral mucosal injuries.