Rapid and Green Fabrication of Nanozyme with Geminal CuN₃O Configuration for Efficient Catecholase‐Mimicking

Abstract Fabrication of nanozyme with catecholase‐like catalytic activity faces the great challenge of merging outstanding activity with low cost as well as simple, rapid, and low‐energy‐consumed production, restricting its industrial applications. Herein, an inexpensive yet robust nanozyme (i.e., DT‐Cu) via simple one‐step coordination between diaminotriazole (DT) and CuSO₄ within 1 h in water at room temperature is constructed. The asymmetric dicopper site with CuN₃O configuration for each copper as well as Cu─O bond length of ≈1.83 Å and Cu···Cu distance of ≈3.5 Å in DT‐Cu resemble those in catechol oxidase (CO), which ensure its prominent intrinsic activity, outperforming most CO‐mimicking nanozymes and artificial homogeneous catalysts. The use of inexpensive DT/CuSO₄ in this one‐pot strategy endows DT‐Cu with only ≈20% cost of natural CO per activity unit. During catalysis, O₂ experienced a 4e‐dominated reduction process accompanied by the formation of ¹O₂ and H₂O₂ intermediates and the product of H₂O. Benefiting from the low cost as well as the distinctive structure and superior intrinsic activity, DT‐Cu presents potential applications ranging from biocatalysis to analytical detection of biomolecules such as epinephrine and beyond.