Engineering Assembly of Plasmonic Virus‐Like Gold SERS Nanoprobe Guided by Intelligent Dual‐Machine Nanodevice for High‐Performance Analysis of Tetracycline

Abstract Accurate detection of trace tetracyclines (TCs) in complex matrices is of great significance for food and environmental safety monitoring. However, traditional recognition and amplification tools exhibit poor specificity and sensitivity. Herein, a novel dual‐machine linkage nanodevice (DMLD) is proposed for the first time to achieve high‐performance analysis of TC, with a padlock aptamer component as the initiation command center, nucleic acid‐encoded multispike virus‐like Au nanoparticles (nMVANs) as the signal indicator, and cascade walkers circuit as the processor. The existence of spike vertices and interspike nanogaps in MVANs enables intense electromagnetic near‐field focusing, allowing distinct surface‐enhanced Raman scattering (SERS) activity. Moreover, through the sequential activation between multistage walker catalytic circuits, the DLMD system converts the limited TC recognition into massive engineering assemblies of SERS probes guided by DNA amplicons, resulting in synergistic enhancement of bulk plasmonic hotspot entities. The continuously guaranteed target recognition and progressively promoted signal enhancement ensure highly specific amplification analysis of TC, with a detection limit as low as 7.94 × 10 ⁻¹⁶ g mL ⁻¹ . Furthermore, the reliable recoveries in real samples confirm the practicability of the proposed sensing platform, highlighting the enormous potential of intelligent nanomachines for analyzing the trace hazards in the environment and food.