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journal article·2022-02Open access

Multiplex SNP Genotyping Using SWITCH: Sequence‐Specific Nanoparticle with Interpretative Toehold‐Mediated Sequence Decoding in Hydrogel

Woongsun Choi, Eunhye Park, Seojin Bae, Kyung‐Hak Choi, Sangeun Han, Kuk‐Hui Son, Do Young Lee, Il‐Joo Cho, Hyejeong Seong, Kyo Seon Hwang, Jwa‐Min Nam, Jungkyu Choi, Hyojin Lee, Nakwon Choi

Small· Volume 18· Issue 8
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Abstract

Abstract Single nucleotide polymorphisms (SNPs) that can alter phenotypes of individuals play a pivotal role in disease development and, more importantly, responses to therapy. However, SNP genotyping has been challenging due to the similarity of SNP alleles and their low concentration in biological samples. Sequence‐specific nanoparticle with interpretative toehold‐mediated sequence decoding in hydrogel (SWITCH) for multiplex SNP genotyping is presented. The encoding with gold nanoparticle probes transduces each SNP target to ≈1000 invaders with prominently different sequences between wild and mutant types, featuring polymerase chain reaction (PCR)‐free amplification. Subsequently, the toehold‐mediated DNA replacement in hydrogel microparticles decodes the invaders via SNP‐specific fluorescence signals. The 4‐plex detection of the warfarin‐associated SNP targets spiked in commercially validated human serum (S1‐100ML, Merck) is successfully demonstrated with excellent specificity. This work is the first technology development presenting PCR‐free, multiplex SNP genotyping with a single reporting fluorophore, to the best of knowledge.
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Research context

PolymersNanomaterials

OpenAlex topics: Advanced biosensing and bioanalysis techniques · Biosensors and Analytical Detection · Advanced Biosensing Techniques and Applications

SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.

Affiliations

Department of Chemical and Biological Engineering Korea University Seoul 02841 Korea

Brain Science Institute Korea Institute of Science and Technology (KIST) Seoul 02792 Korea

Department of Chemistry Seoul National University Seoul 08826 Korea

Biomaterials Research Center Biomedical Research Division Korea Institute of Science and Technology (KIST) Seoul 02792 Korea

OPTOLANE Technologies, Inc. Seongnam 13494 Korea

Department of Thoracic and Cardiovascular Surgery Gachon University Gil Medical Center Incheon 21936 Korea

Yonsei‐KIST Convergence Research Institute Yonsei University Seoul 03722 Korea

School of Electrical and Electronics Engineering Yonsei University Seoul 03722 Korea

Division of Bio‐Medical Science & Technology KIST School Korea University of Science and Technology (UST) Seoul 02792 Korea

Department of Clinical Pharmacology and Therapeutics College of Medicine Kyung Hee University Seoul 02447 Korea

KU‐KIST Graduate School of Converging Science and Technology Korea University Seoul 02841 Korea

Funding

National Research Foundation of Korea

References (47)

Show source-provided references
1. 10.1101/gr.8.8.76910.1101/gr.8.8.769 ↗
2. 10.1016/j.mrfmmm.2005.01.00510.1016/j.mrfmmm.2005.01.005 ↗
3. 10.1016/j.forsciint.2004.10.02010.1016/j.forsciint.2004.10.020 ↗
4. 10.1016/j.forsciint.2005.11.01710.1016/j.forsciint.2005.11.017 ↗
5. Parmacogenomics: What does it mean for your health? https://www.cdc.gov/genomics/disease/pharma.htm(accessed: October 2018).
6. Alwi Z. B. · Malays. J. Sci. · 2005 · 12 · 4
7. 10.1111/j.1365-2516.2010.02291.x10.1111/j.1365-2516.2010.02291.x ↗
8. 10.1182/blood-2011-12-39775210.1182/blood-2011-12-397752 ↗
9. 10.1038/sj.clpt.610008710.1038/sj.clpt.6100087 ↗
10. 10.2353/jmoldx.2009.08012310.2353/jmoldx.2009.080123 ↗
11. Shen G.‐Q. · 2009 · 29310.1007/978-1-60327-411-1_19 ↗
12. 10.1007/978-1-61737-954-3_610.1007/978-1-61737-954-3_6 ↗
13. 10.2144/03345dd0310.2144/03345dd03 ↗
14. 10.1007/978-1-60327-411-1_2610.1007/978-1-60327-411-1_26 ↗
15. 10.1007/s11032-013-9917-x10.1007/s11032-013-9917-x ↗
16. 10.1371/journal.pone.021722210.1371/journal.pone.0217222 ↗
17. 10.1073/pnas.89.2.49510.1073/pnas.89.2.495 ↗
18. 10.1016/j.msec.2006.09.00810.1016/j.msec.2006.09.008 ↗
19. 10.1016/j.snb.2017.04.06610.1016/j.snb.2017.04.066 ↗
20. 10.1016/j.biomaterials.2017.04.04910.1016/j.biomaterials.2017.04.049 ↗
21. 10.1007/s10038-005-0354-510.1007/s10038-005-0354-5 ↗
22. 10.1038/clpt.2010.32210.1038/clpt.2010.322 ↗
23. 10.1111/j.1582-4934.2012.01606.x10.1111/j.1582-4934.2012.01606.x ↗
24. 10.1039/C2AY25904D10.1039/C2AY25904D ↗
25. 10.1021/ac504869610.1021/ac5048696 ↗
26. 10.1016/j.eurpolymj.2015.02.02210.1016/j.eurpolymj.2015.02.022 ↗
27. 10.1016/j.bios.2015.12.06810.1016/j.bios.2015.12.068 ↗
28. 10.1038/ncomms632410.1038/ncomms6324 ↗
29. 10.1021/acsnano.7b0867310.1021/acsnano.7b08673 ↗
30. 10.1021/ja906987s10.1021/ja906987s ↗
31. 10.1002/smll.20100090810.1002/smll.201000908 ↗
32. 10.1021/ja310991r10.1021/ja310991r ↗
33. 10.1016/j.bios.2012.09.02310.1016/j.bios.2012.09.023 ↗
34. 10.1021/jacs.1c0056810.1021/jacs.1c00568 ↗
35. 10.1021/ac302128u10.1021/ac302128u ↗
36. 10.1021/acs.analchem.5b0419010.1021/acs.analchem.5b04190 ↗
37. 10.1039/C8LC01160E10.1039/C8LC01160E ↗
38. 10.1016/j.aca.2019.05.00910.1016/j.aca.2019.05.009 ↗
39. 10.1021/ac900529210.1021/ac9005292 ↗
40. Marras S. A. · 2006 · 310.1385/1-59745-069-3:3 ↗
41. 10.1073/pnas.180685911510.1073/pnas.1806859115 ↗
42. 10.1016/j.jbiotec.2007.06.02310.1016/j.jbiotec.2007.06.023 ↗
43. 10.1016/0003-2697(88)90047-410.1016/0003-2697(88)90047-4 ↗
44. 10.1093/nar/22.21.454310.1093/nar/22.21.4543 ↗
45. 10.1002/jgm.51210.1002/jgm.512 ↗
46. 10.1039/C6AN00188B10.1039/C6AN00188B ↗
47. Jacobs M. N. · Altern. Anim. Exp. · 2019 · 36 · 163