Laminin‐Augmented Decellularized Extracellular Matrix Ameliorating Neural Differentiation and Neuroinflammation in Human Mini‐Brains
Source abstract
Abstract
Abstract Non‐neural extracellular matrix (ECM) has limited application in humanized physiological neural modeling due to insufficient brain‐specificity and safety concerns. Although brain‐derived ECM contains enriched neural components, certain essential components are partially lost during the decellularization process, necessitating augmentation. Here, it is demonstrated that the laminin‐augmented porcine brain‐decellularized ECM (P‐BdECM) is xenogeneic factor‐depleted as well as favorable for the regulation of human neurons, astrocytes, and microglia. P‐BdECM composition is comparable to human BdECM regarding brain‐specificity through the matrisome and gene ontology‐biological process analysis. As augmenting strategy, laminin 111 supplement promotes neural function by synergic effect with laminin 521 in P‐BdECM. Annexin A1(ANXA1) and Peroxiredoxin(PRDX) in P‐BdECM stabilized microglial and astrocytic behavior under normal while promoting active neuroinflammation in response to neuropathological factors. Further, supplementation of the brain‐specific molecule to non‐neural matrix also ameliorated glial cell inflammation as in P‐BdECM. In conclusion, P‐BdECM‐augmentation strategy can be used to recapitulate humanized pathophysiological cerebral environments for neurological study.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Tissue Engineering and Regenerative Medicine · Neuroinflammation and Neurodegeneration Mechanisms · Nerve injury and regeneration
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) Pohang Kyungbuk 37673 South Korea
Department of Biophysics Institute of Quantum Biophysics Department of Intelligent Precision Healthcare Convergence Sungkyunkwan University Suwon Gyeonggi 16419 South Korea
Biomedical Research Institute Chonnam National University Hospital Gwangju 61469 South Korea
School of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology (POSTECH) Pohang Kyungbuk 37673 South Korea
Department of Convergence IT Engineering Pohang University of Science and Technology (POSTECH) Pohang Kyungbuk 37673 South Korea
Department of Convergence Biosystems Engineering College of Agriculture and Life Sciences Chonnam National University Gwangju 61186 South Korea
Department of Forensic medicine Chonnam National University Medical School & Research Institute of Medical Sciences Gwangju 61469 South Korea
Institute for Convergence Research and Education in Advanced Technology Yonsei University Seoul 03722 Republic of Korea
Funding
Korea Dementia Research Center · Ministry of Science and ICT, South Korea · Arthritis National Research Foundation
References (98)
Show source-provided references
1. 10.1002/glia.2283910.1002/glia.22839 ↗
2. 10.3389/fnano.2021.64350710.3389/fnano.2021.643507 ↗
3. 10.1038/s42003-021-02910-810.1038/s42003-021-02910-8 ↗
4. 10.3389/fncel.2015.0037710.3389/fncel.2015.00377 ↗
5. 10.1016/j.biomaterials.2014.11.04610.1016/j.biomaterials.2014.11.046 ↗
6. 10.1002/adhm.20190141910.1002/adhm.201901419 ↗
7. 10.1038/s41467-022-29279-410.1038/s41467-022-29279-4 ↗
8. 10.1038/s41578-020-0199-810.1038/s41578-020-0199-8 ↗
9. 10.1089/ten.tea.2010.072410.1089/ten.tea.2010.0724 ↗
10. 10.1038/s41551-018-0260-810.1038/s41551-018-0260-8 ↗
11. 10.1038/s41467-020-20314-w10.1038/s41467-020-20314-w ↗
12. 10.1038/s41551-019-0363-x10.1038/s41551-019-0363-x ↗
13. 10.1371/journal.pone.024568510.1371/journal.pone.0245685 ↗
14. 10.1016/j.jmst.2022.08.02810.1016/j.jmst.2022.08.028 ↗
15. 10.1088/1758-5090/ac293f10.1088/1758-5090/ac293f ↗
16. 10.3390/life1106053810.3390/life11060538 ↗
17. 10.1021/acs.chemrev.9b0080810.1021/acs.chemrev.9b00808 ↗
18. 10.3389/fbioe.2020.59509610.3389/fbioe.2020.595096 ↗
19. 10.1007/s10561-017-9619-410.1007/s10561-017-9619-4 ↗
20. 10.1002/term.292310.1002/term.2923 ↗
21. 10.1007/s13770-021-00419-z10.1007/s13770-021-00419-z ↗
22. 10.1016/j.biomaterials.2011.01.05710.1016/j.biomaterials.2011.01.057 ↗
23. 10.1038/ncomms493510.1038/ncomms4935 ↗
24. 10.1016/j.bioactmat.2021.12.00510.1016/j.bioactmat.2021.12.005 ↗
25. Landreth G. · 1999 · 473
26. 10.1126/science.aay594710.1126/science.aay5947 ↗
27. 10.1093/glycob/6.5.48910.1093/glycob/6.5.489 ↗
28. Karsdal M. · 2019
29. 10.1016/j.celrep.2017.10.02810.1016/j.celrep.2017.10.028 ↗
30. 10.1002/dneu.2205710.1002/dneu.22057 ↗
31. 10.4049/jimmunol.100109510.4049/jimmunol.1001095 ↗
32. 10.1016/j.jneuroim.2013.03.00610.1016/j.jneuroim.2013.03.006 ↗
33. 10.1016/j.cjtee.2020.02.00210.1016/j.cjtee.2020.02.002 ↗
34. 10.1126/science.288.5463.15010.1126/science.288.5463.150 ↗
35. 10.1111/j.1365-2443.2005.00893.x10.1111/j.1365-2443.2005.00893.x ↗
36. 10.1038/s41598-018-37186-210.1038/s41598-018-37186-2 ↗
37. 10.1016/j.neulet.2014.10.01610.1016/j.neulet.2014.10.016 ↗
38. 10.1101/cshperspect.a00165110.1101/cshperspect.a001651 ↗
39. 10.1186/s12974-019-1459-710.1186/s12974-019-1459-7 ↗
40. Yao J. · Mol. Psychiatry · 2023 · 1
41. 10.1186/s13024-023-00616-510.1186/s13024-023-00616-5 ↗
42. 10.1111/jnc.1468710.1111/jnc.14687 ↗
43. 10.1186/s12974-016-0692-610.1186/s12974-016-0692-6 ↗
44. 10.1016/j.tips.2007.12.00310.1016/j.tips.2007.12.003 ↗
45. 10.1016/j.cyto.2008.01.00610.1016/j.cyto.2008.01.006 ↗
46. 10.1186/s12974-015-0434-110.1186/s12974-015-0434-1 ↗
47. 10.1186/s12974-019-1643-910.1186/s12974-019-1643-9 ↗
48. 10.1146/annurev.immunol.23.021704.11565310.1146/annurev.immunol.23.021704.115653 ↗
49. 10.1074/jbc.M60589820010.1074/jbc.M605898200 ↗
50. 10.1074/jbc.M11188320010.1074/jbc.M111883200 ↗
51. 10.1016/j.cell.2017.05.01810.1016/j.cell.2017.05.018 ↗
52. 10.1016/j.conb.2022.10255010.1016/j.conb.2022.102550 ↗
53. 10.1093/jnen/nlz07910.1093/jnen/nlz079 ↗
54. 10.1074/jbc.M112.37710110.1074/jbc.M112.377101 ↗
55. 10.1038/nrrheum.2013.12610.1038/nrrheum.2013.126 ↗
56. 10.1186/s40478-023-01526-910.1186/s40478-023-01526-9 ↗
57. 10.1002/glia.2109410.1002/glia.21094 ↗
58. Eberhard D. A. · Am. J. Pathol. · 1994 · 145 · 640
59. 10.1111/j.1742-4658.2009.06985.x10.1111/j.1742-4658.2009.06985.x ↗
60. 10.3390/antiox912120310.3390/antiox9121203 ↗
61. 10.1016/j.neulet.2005.02.04810.1016/j.neulet.2005.02.048 ↗
62. 10.3389/fnins.2020.0018110.3389/fnins.2020.00181 ↗
63. U. S. D. o. H. a. H. Services F. a. D. Administration C. f. D. a. R. Health U.S. Food and Drug Administration2019.
64. 10.1016/j.biomaterials.2010.07.08310.1016/j.biomaterials.2010.07.083 ↗
65. 10.3109/02713683.2015.106211410.3109/02713683.2015.1062114 ↗
66. 10.1177/00221554000480060310.1177/002215540004800603 ↗
67. 10.1038/sj.bjc.660392310.1038/sj.bjc.6603923 ↗
68. 10.1186/s12974-016-0503-010.1186/s12974-016-0503-0 ↗
69. 10.1016/j.celrep.2016.11.06810.1016/j.celrep.2016.11.068 ↗
70. 10.1038/s41598-018-20463-510.1038/s41598-018-20463-5 ↗
71. 10.3389/fendo.2019.0082210.3389/fendo.2019.00822 ↗
72. 10.1016/S1534-5807(03)00128-X10.1016/S1534-5807(03)00128-X ↗
73. 10.1006/excr.1996.001810.1006/excr.1996.0018 ↗
74. 10.1016/j.addr.2015.11.00110.1016/j.addr.2015.11.001 ↗
75. 10.1007/s00109-007-0182-510.1007/s00109-007-0182-5 ↗
76. 10.1016/j.scr.2017.09.00210.1016/j.scr.2017.09.002 ↗
77. 10.1111/brv.1245410.1111/brv.12454 ↗
78. Sun G. · Cell Rep. · 2021 · 35
79. 10.1016/j.matbio.2005.12.00110.1016/j.matbio.2005.12.001 ↗
80. 10.1074/jbc.272.21.1390410.1074/jbc.272.21.13904 ↗
81. Sugimoto M. A. · J. Immunol. Res. · 2016 · 2016
82. 10.3389/fimmu.2012.0035410.3389/fimmu.2012.00354 ↗
83. 10.1016/S0197-4580(03)00126-X10.1016/S0197-4580(03)00126-X ↗
84. 10.1186/1742-2094-8-7910.1186/1742-2094-8-79 ↗
85. 10.1111/imm.1345510.1111/imm.13455 ↗
86. 10.3390/cells1009224510.3390/cells10092245 ↗
87. 10.1186/s12974-021-02174-310.1186/s12974-021-02174-3 ↗
88. 10.1016/j.it.2016.06.00610.1016/j.it.2016.06.006 ↗
89. 10.1002/jcb.2441210.1002/jcb.24412 ↗
90. 10.1523/JNEUROSCI.4589-10.201110.1523/JNEUROSCI.4589-10.2011 ↗
91. 10.1007/s11064-020-02971-x10.1007/s11064-020-02971-x ↗
92. 10.1016/j.redox.2022.10234710.1016/j.redox.2022.102347 ↗
93. 10.18632/oncotarget.1877310.18632/oncotarget.18773 ↗
94. 10.4049/jimmunol.090194510.4049/jimmunol.0901945 ↗
95. 10.1038/s41593-018-0175-410.1038/s41593-018-0175-4 ↗
96. 10.1002/advs.20210125110.1002/advs.202101251 ↗
97. 10.3390/ijms2112423610.3390/ijms21124236 ↗
98. 10.3390/biomedicines811045510.3390/biomedicines8110455 ↗