An Automated Single‐Cell Droplet‐Digital Microfluidic Platform for Monoclonal Antibody Discovery
Source abstract
Abstract
Abstract Monoclonal antibody (mAb) discovery plays a prominent role in diagnostic and therapeutic applications. Droplet microfluidics has become a standard technology for high‐throughput screening of antibody‐producing cells due to high droplet single‐cell confinement frequency and rapid analysis and sorting of the cells of interest with their secreted mAbs. In this work, a new method is described for on‐demand co‐encapsulation of cells that eliminates the difficulties associated with washing in between consecutive steps inside the droplets and enables the washing and addition of fresh media. The new platform identifies hybridoma cells that are expressing antibodies of interest using antibody‐characterization assays to find the best‐performing or rare‐cell antibody candidates.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Innovative Microfluidic and Catalytic Techniques Innovation · Electrowetting and Microfluidic Technologies · Microfluidic and Bio-sensing Technologies
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
Department of Electrical and Computer Engineering Concordia University 1455 de Maisonneuve Blvd. West Montréal Québec H3G 1M8 Canada
Centre for Applied Synthetic Biology Concordia University 7141 Sherbrooke Street West Montréal Québec H4B 1R6 Canada
Department of Biology Concordia University 7141 Sherbrooke Street West Montréal Québec H4B 1R6 Canada
Human Health Therapeutics Research Centre National Research Council Canada Montréal Québec H4P 2R2 Canada
Funding
Natural Sciences and Engineering Research Council of Canada
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