De Novo Designed Peptide and Protein Hairpins Self‐Assemble into Sheets and Nanoparticles
Source abstract
Abstract
Abstract The design and assembly of peptide‐based materials has advanced considerably, leading to a variety of fibrous, sheet, and nanoparticle structures. A remaining challenge is to account for and control different possible supramolecular outcomes accessible to the same or similar peptide building blocks. Here a de novo peptide system is presented that forms nanoparticles or sheets depending on the strategic placement of a “disulfide pin” between two elements of secondary structure that drive self‐assembly. Specifically, homodimerizing and homotrimerizing de novo coiled‐coil α‐helices are joined with a flexible linker to generate a series of linear peptides. The helices are pinned back‐to‐back, constraining them as hairpins by a disulfide bond placed either proximal or distal to the linker. Computational modeling indicates, and advanced microscopy shows, that the proximally pinned hairpins self‐assemble into nanoparticles, whereas the distally pinned constructs form sheets. These peptides can be made synthetically or recombinantly to allow both chemical modifications and the introduction of whole protein cargoes as required.
Source: crossref · Quality: baseline
Research context
OpenAlex topics: Supramolecular Self-Assembly in Materials · Chemical Synthesis and Analysis · Advanced biosensing and bioanalysis techniques
SciVX discovery tags are terminology-based. OpenAlex topics are source-provided classifications.
Affiliations
School of Chemistry University of Bristol Cantock's Close Bristol BS8 1TS UK
School of Chemistry University of Leeds Leeds LS2 9JT UK
School of Biochemistry University of Bristol Medical Sciences Building University Walk Bristol BS8 1TD UK
BrisSynBio/Bristol Biodesign Institute University of Bristol Life Sciences Building, Tyndall Avenue Bristol BS8 1TQ UK
Bristol Centre for Functional Nanomaterials School of Physics University of Bristol HH Wills Physics Laboratory Tyndall Avenue Bristol BS8 1TL UK
School of Biological and Chemical Sciences Fogg Building Queen Mary University of London Mile End Road London E1 4QD UK
School of Chemistry University of Glasgow 0/1 125 Novar Drive Glasgow G12 9TA UK
School of Biological Sciences Roger Land Building, King's Buildings Edinburgh EH9 3JQ UK
Funding
Engineering and Physical Sciences Research Council · Biotechnology and Biological Sciences Research Council
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