Facile Surface Functionalization Strategy for Two‐Photon Lithography Microstructures

Abstract Two‐photon lithography (TPL) is a powerful tool to construct small‐scale objects with complex and precise 3D architectures. While the limited selection of chemical functionalities on the printed structures has restricted the application of this method in fabricating functional objects and devices, this study presents a facile, efficient, and extensively applicable method to functionalize the surfaces of the objects printed by TPL. TPL‐printed objects, regardless of their compositions, can be efficiently functionalized by combining trichlorovinylsilane treatment and thiol–ene chemistry. Various functionalities can be introduced on the printed objects, without affecting their micro‐nano topographies. Hence, microstructures with diverse functions can be generated using non‐functional photoresists. Compared to existed strategies, this method is fast, highly efficient, and non photoresist‐dependent. In addition, this method can be applied to various materials, such as metals, metal oxides, and plastics that can be potentially utilized in TPL or other 3D printing technologies. The applications of this method on the biofunctionalization of microrobots and cell scaffolds are also demonstrated.