FluorMango, an RNA‐Based Fluorogenic Biosensor for the Direct and Specific Detection of Fluoride

Abstract Nucleic acids are not only essential actors of cell life but also extremely appealing molecular objects in the development of synthetic molecules for biotechnological application, such as biosensors to report on the presence and concentration of a target ligand by emission of a measurable signal. In this work, FluorMango, a fluorogenic ribonucleic acid (RNA)‐based biosensor specific for fluoride is introduced. The molecule consists of two RNA aptamer modules, a fluoride‐specific sensor derived from the crcB riboswitch which changes its structure upon interaction with the target ion, and the light‐up RNA Mango‐III that emits fluorescence when complexed with a fluorogen. The two modules are connected by an optimized communication module identified by ultrahigh‐throughput screening, which results in extremely high fluorescence of FluorMango in the presence of fluoride, and background fluorescence in its absence. The value and efficiency of this biosensor by direct monitoring of defluorinase activity in living bacterial cells is illustrated, and the use of this new tool in future screening campaigns aiming at discovering new defluorinase activities is discussed.